Three enzymes are combined under mild conditions for the preparative scale oxidation of HMF to FDCA and a range of 10 alcohols.

One-pot multienzyme continuous cascade for the conversion of HMF to FDCA using HRP to activate GOaseM3-5 in situ.

All chemical reagents were of analytical grade and were used with no additional purification unless otherwise stated. Uric acid (≥99%), hydroxylamine hydrochloride (99%), and water (HPLC grade) were purchased from Sigma Aldrich Ltd. (Dorset, UK). Potassium dihydrogen phosphate, dipotassium phosphate, sodium hydroxide, silver nitrate, hydrochloric acid and nitric acid were of analytical grade and obtained from Fischer Scientific (Loughborough, UK). Clinical pre-preeclampsia urine samples were kindly donated by Professor B.