A recombinant integrin expression system has been created for the large-scale production of αVβ5 integrin extracellular domains that take advantage of Fos and Jun dimerization for expression in bacterial, insect, and mammalian cells. This utilizes an all-in-one vector, pQE-TriSystem, with molecular machinery for parallel expression without the need of additional subcloning. Optimal expression in HEK293 cells was determined by a time course analysis. The heterodimer was purified in a one-step nickel column purification scheme, and the sequence and functional state were confirmed by mass spectrometry and inhibition assays, respectively. The yields of αVβ5 integrin obtained are in quantities suitable for multiple applications including structural biology and functional assays.
A previously healthy young man in his late teens presented to his dentist with a 3-week history of left-sided facial pain, fullness, and trismus. He underwent a third maxillary molar extraction and attempted incision and drainage of the area with no improvement in symptoms. No purulence or clinically significant bleeding was identified. A contrast-enhanced computed tomographic (CT) scan was performed (Figure , A), and the patient was referred to the otolaryngology clinic. On presentation, he also reported decreased acuity in his left lower visual field, continued swelling with increasing pain, and worsening trismus. Physical examination revealed mild tenderness over a mass that extended inferiorly to the zygoma into the buccal cavity and posterolaterally to the left superior molars. The patient also reported hypoesthesia of the left maxillary division of the trigeminal nerve. Fiberoptic scope of the nasopharynx revealed a small lesion posterior to the left middle turbinate. Contrast-enhanced magnetic resonance imaging of his head, neck, and brain was performed (Figure, B and C).
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