The contribution of gap junctions to endothelium‐dependent relaxation was investigated in isolated rabbit conduit artery preparations pre‐constricted by 10 μM phenylephrine (PhE). Acetylcholine (ACh) relaxed the thoracic aorta by ≈60 % and the superior mesenteric artery (SMA) by ≈90 %. A peptide possessing sequence homology with extracellular loop 2 of connexin 43 (Gap 27, 300 μM) inhibited relaxation by ≈40 % in both artery types. Gap 27 also attenuated the endothelium‐dependent component of the relaxation induced by ATP in thoracic aorta but did not modify force development in response to PhE. N G‐nitro‐L‐arginine methyl ester (L‐NAME, 300 μM), an inhibitor of NO synthase, attenuated ACh‐induced relaxation by ≈90 % in the aorta but only by ≈40 % in SMA (P < 0.05). Residual L‐NAME‐insensitive relaxations were almost abolished by 300 μM Gap 27 in aorta and inhibited in a concentration‐dependent fashion in SMA (≈50 % at 100 μM and ≈80 % at 10 mM). Gap 27 similarly attenuated the endothelium‐dependent component of L‐NAME‐insensitive relaxations to ATP in aorta. Responses to cyclopiazonic acid, which stimulates endothelium‐dependent relaxation through a receptor‐independent mechanism, were also attenuated by Gap 27, whereas this peptide exerted no effect on the NO‐mediated relaxation induced by sodium nitroprusside in preparations denuded of endothelium. ACh‐induced relaxation of ‘sandwich’ mounts of aorta or SMA were unaffected by Gap 27 but completely abolished by L‐NAME. We conclude that direct heterocellular communication between the endothelium and smooth muscle contributes to endothelium‐dependent relaxations evoked by both receptor‐dependent and ‐independent mechanisms. The inhibitory effects of Gap 27 peptide do not involve homocellular communication within the vessel wall or modulation of NO release or action.
Phenylephrine (10 μM) evoked rises in tension in isolated rings of endothelium‐denuded rabbit superior mesenteric artery. These increases consisted of a tonic component with superimposed rhythmic activity, the frequency of which generally remained constant over time but whose amplitude exhibited cycle‐to‐cycle variability. The amplitude, but not the frequency, of the rhythmic activity was affected by a series of short peptides possessing sequence homology with extracellular loops 1 and 2 of connexin 43 (Cx43). Oscillatory behaviour was abolished at concentrations of 100–300 μM (IC50 of 20–30 μM), without change in average tone. No synergy was evident between peptides corresuponding to the extracellular loops, and cytoplasmic loop peptides were biologically inactive. The putative gap junction inhibitor heptanol mimicked the action of the extracellular loop peptides and abolished rhythmic activity at concentrations of 100–300 μM without effects on frequency. However, in marked contrast to the peptides, heptanol completely inhibited the contraction evoked by phenylephrine (IC50, 283 ± 28 μM). The presence of mRNA encoding Cx32, Cx40 and Cx43 was detected in the rabbit superior mesenteric artery by reverse transcriptase‐polymerase chain reaction. Western blot analysis showed that Cx43 was the major connexin in the endothelium‐denuded vessel wall. We conclude that intercellular communication between vascular smooth muscle cells via gap junctions is essential for synchronized rhythmic activity in isolated arterial tissue, whereas tonic force development appears to be independent of cell‐cell coupling. The molecular specificity of the peptide probes employed in the study suggests that the smooth muscle relaxant effects of heptanol may be non‐supecific and unrelated to inhibition of gap junctional communication.
An endothelium-derived hyperpolarizing factor (EDHF) is now widely recognized to mediate endothelium-dependent vascular relaxations that are independent of nitric oxide (NO) and prostanoid synthesis (Mombouli & Vanhoutte, 1997). Although the chemical identity of EDHF remains controversial, there is accumulating evidence that this mediator normally effects relaxation following diffusion from the endothelium to smooth muscle via myoendothelial gap junctions rather than the extracellular space Taylor et al. 1998;Dora et al. 1999;Hutcheson et al. 1999). Anatomically, this hypothesis is supported by the demonstration of myoendothelial gap junction plaques in rabbit conduit arteries (Spagnoli et al. 1982), and functional dye transfer experiments confirm direct chemical coupling between endothelium and subjacent smooth muscle (Little et al. 1995). Gap junctions are membrane structural proteins which consist of two hemichannels or connexons contributed by apposing cells, with each connexon being formed from six protein subunits or connexins arranged around an aqueous central pore that permits intercellular transfer of electrical current and molecules < 1 kDa in size (Yeager & Nicholson, 1996). Connexin 43 (Cx43) is present in both endothelial and vascular smooth muscle cells, and EDHFmediated relaxations and hyperpolarizations of rabbit
Synthetic peptides homologous to the Gap 26 and Gap 27 domains of the first and second extracellular loops of the major vascular connexins (Cx37, Cx40, and Cx43) have been used to investigate the role of gap junctions in endothelium-derived hyperpolarizing factor (EDHF)-type relaxations of the rat hepatic artery. These peptides were designated 37,40Gap 26, 43Gap 26, 37,43Gap 27, and 40Gap 27, according to connexin specificity. When administered at 600 microM, none of the peptides individually affected maximal EDHF-type relaxations to ACh. By contrast, at 300 microM each, paired peptide combinations targeting more than one connexin subtype attenuated relaxation by up to 50%, and responses were abolished by the triple peptide combination 43Gap 26 + 40Gap 27 + 37,43Gap 27. In parallel experiments with A7r5 cells expressing Cx40 and Cx43, neither 43Gap 26 nor 40Gap 27 affected intercellular diffusion of Lucifer yellow individually but, in combination, significantly attenuated dye transfer. The findings confirm that functional cell-cell coupling may depend on more than one connexin subtype and demonstrate that direct intercellular communication via gap junctions constructed from Cx37, Cx40, and Cx43 underpins EDHF-type responses in the rat hepatic artery.
We have investigated the role of cAMP in NO-and prostanoidindependent relaxations that are widely attributed to an endothelium-derived hyperpolarizing factor (EDHF). Under control conditions EDHF-type relaxations evoked by acetylcholine (ACh) in rabbit iliac arteries were transient, but in the presence of the cAMP phosphodiesterase inhibitor isobutylmethylxanthine (IBMX) or the cell permeant cAMP analog 8-bromo-cAMP, relaxations became sustained with their maxima potentiated Ϸ2-fold. Relaxation was associated with transient Ϸ1.5-fold elevations in smooth muscle cAMP levels with both mechanical and nucleotide responses being abolished by interrupting gap junctional communication with the connexin-mimetic peptide Gap 27 and by endothelial denudation. However, IBMX induced a sustained endothelium-independent Ϸ2-fold rise in cAMP levels, which was not further amplified by ACh, suggesting that the contribution of cAMP to the EDHF phenomenon is permissive. After selective loading of the endothelium with calcein AM, direct transfer of dye from the endothelium to the media was enhanced by IBMX or 8-bromo-cAMP, but not by 8-bromo-cGMP, whereas Gap 27 promoted sequestration within the intima. ACh-induced hyperpolarizations of subintimal smooth muscle in arterial strips with intact endothelium were abolished by Gap 27 and the adenylyl cyclase inhibitor 2 ,5 -dideoxyadenosine but were unaffected by IBMX. By contrast, in strips partially denuded of endothelium, IBMX enhanced the transmission of hyperpolarization from the endothelium to remote smooth muscle cells. These findings support the hypothesis that endothelial hyperpolarization underpins the EDHF phenomenon, with cAMP governing subsequent electrotonic signaling via both myoendothelial and homocellular smooth muscle gap junctions.connexin ͉ acetylcholine ͉ IBMX ͉ 8-bromo-cAMP ͉ cGMP
NO- and prostanoid-independent relaxations are generally assumed to be mediated by an endothelium-derived hyperpolarizing factor (EDHF) that has been postulated to be an arachidonic acid metabolite. Recent evidence also suggests that direct heterocellular gap junctional communication (GJC) between endothelium and smooth muscle contributes to NO-independent relaxations. In the present study we have investigated the contribution of phospholipase A2 (PLA2)-linked metabolites and GJC to EDHF-type relaxations in rabbit mesenteric artery. In isolated rings preconstricted with 10 micromol/L phenylephrine in the presence of NG-nitro-L-arginine methyl ester (L-NAME) and indomethacin, acetylcholine (ACh) and the Ca2+ ionophore A23187 evoked relaxations that were markedly attenuated by the Ca2+-dependent PLA2 inhibitors 2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid (3 micromol/L) and arachidonyl trifluoromethyl ketone (3 micromol/L), but were potentiated by the sulfhydryl agent thimerosal (300 nmol/L). In intact rings, relaxations to ACh were attenuated synergistically by L-NAME and Gap 27 peptide, an inhibitor of GJC, whereas ACh-evoked relaxations of "sandwich" preparations were unaffected by the peptide but were abolished by L-NAME. In both ring and sandwich preparations A23187-induced relaxations were attenuated by inhibition of PLA2 but were insensitive to L-NAME and Gap 27 peptide. We conclude that EDHF-type relaxations of rabbit mesenteric artery to ACh and A23187 depend on a common pathway that involves activation of PLA2. In the case of ACh, relaxation requires transfer of a factor or factors from the endothelium to smooth muscle via gap junctions, whereas A23187 permits release directly into the extracellular space.
The gap junction inhibitor 18-a-glycyrrhetinic acid (a-GA, 100 mM) attenuated endothelium-dependent relaxations to acetylcholine and cyclopiazonic acid by *20% in rings of pre-constricted rabbit iliac artery. The nitric oxide synthase inhibitor N G -nitro-L-arginine methyl ester (L-NAME, 300 mM) inhibited relaxations to both agents by *65% and these were further attenuated by a-GA to 510% of control. In endothelium-denuded preparations, relaxations to sodium nitroprusside were not a ected by a-GA. Heterocellular gap junctional communication may therefore account for nitric oxide-independent relaxations evoked both by receptor-dependent and -independent mechanisms in rabbit iliac artery.
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