Versão corrigida. A versão original eletrônica se encontra-se disponível tanto na Biblioteca do ICB quanto na Biblioteca Digital de Teses e Dissertações da USP (BDTD). São Paulo 2014À Deus dedico meu trabalho e minha vida. AGRADECIMENTOSAgradeço em primeiro lugar a Deus, que me guiou até aqui, que me mostrou o caminho, que me deu forças quando mais precisava e me deu sabedoria e inteligência durante todas as etapas desse projeto.Ao meu amado esposo Joel, que me apoiou em uma jornada incerta, mas que culminou na realização desse desejo tão especial. Agradeço por me incentivar a ir atrás dos meus sonhos. Por me apoiar. Por secar minhas lágrimas. Por me lembrar de que sempre há uma saída. Agradeço por abrir mão dos seus sonhos para que eu pudesse realizar os meus.Sem essa pessoa tão especial nada disso seria possível.Agradeço aos meus pais, por todo apoio e por serem a base de toda minha vida. Plateletpheresis is an automatized procedure to obtain high purity platelet for transfusions. From this procedure it's possible to obtain a byproduct: The Leukoreduction System Chamber (LRSC), which is discarded at the end of donation process. This type of donation allows 24 donation/year, but the consequences of frequent donations are poorly investigated. Therefore, we identified and quantified leukocytes of frequent and first time platelet donor. Also, was evaluated the viability, for research, of mononuclear cells recovery from LRSC. The total number of mononuclear cells was higher in LRSC than in peripheral blood samples, but the frequencies were similar in both the samples. Activation state and functional capacity (measured by cell proliferation and cytokine production) were similar in both, blood and LRSC mononuclear cells, as well as spontaneous apoptosis. Among frequent (6 or more donations in 1 year) and first time donor, there was no difference in the leukocyte total number, suggesting that frequent donation do not modify these cells.
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