Changes in the central and peripheral serotonergic receptor activity have been reported to be involved in depression and suicidality. To elucidate the interdependence between central and peripheral receptor sites and their regulation by serotonin, we estimated intra-individual serotonin2 receptor binding characteristics in porcine cortex synaptosomes and in platelet membranes using 3H-LSD as ligand and ketanserin as competitor and quantified the relevant serotonin concentrations. A positive correlation between the apparent half maximal saturation concentration, KD, of the receptor in cortex synaptosomes and platelet membranes (r = 0.65, p = 0.0046, n = 18), and between the apparent maximal binding capacity, Bmax, of the receptor in cortex synaptosomes and platelets (r = 0.52, p = 0.027, n = 18) was observed. The blood serotonin concentrations correlated negatively with the maximal binding capacity, Bmax, in platelets (r = -0.77, p = 0.0002, n = 18). These results suggest that the binding characteristics of the central and peripheral serotonin2 receptor are similar, and that the platelet receptor activity may be regulated by blood levels of serotonin.
The present paper constitutes a preliminary report on the results of the study of the karyotype in Vulpes vulpes and Vulpes lagopus.The material of the investigation consisted of three testes from V. vulpes and one testis from V. lagopus, 1) the first fixed by the Zenker-formol, the second by the method of Painter (fixations with Flemming and Hermann fluids).Sections (8-10y) were stained with iron haematoxylin; also toluidin-blue according to the method of Michaelis and gentiana-violet according to the method of Newton; and Feulgen reaction was used throughout.Unfortunately the testes were obtained from the animals at the end of the heat period (in February), so that spermatogenesis was nearly over. Among normal cell divisions many disturbed mitoses were definitely noted.Parts of the seminal tubules, particularly in V. lagopus, seemed blocked up with cell debris or were entirely non-functioning.Only the first meiotic metaphase was investigated in V. vulpes. In all the cells 17 tetrads were found.16 of these were homologous The heteromorphic one in Fig. 2 lies at the side.
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