The activation of heterotrimeric G proteins is accomplished primarily by the guanine nucleotide exchange activity of ligand-bound G protein-coupled receptors. The existence of nonreceptor guanine nucleotide exchange factors for G proteins has also been postulated. Yeast two-hybrid screens with G␣ o and G␣ s as baits were performed to identify binding partners of these proteins. Two mammalian homologs of the Caenorhabditis elegans protein Ric-8 were identified in these screens: Ric-8A (Ric-8/synembryn) and Ric-8B. Purification and biochemical characterization of recombinant Ric-8A revealed that it is a potent guanine nucleotide exchange factor for a subset of G␣ proteins including G␣ q , G␣ i1 , and G␣ o , but not G␣ s . The mechanism of Ric-8A-mediated guanine nucleotide exchange was elucidated. Ric-8A interacts with GDP-bound G␣ proteins, stimulates release of GDP, and forms a stable nucleotide-free transition state complex with the G␣ protein; this complex dissociates upon binding of GTP to G␣.Heterotrimeric guanine nucleotide-binding regulatory proteins mediate signal transduction between many membranebound receptors and intracellular effectors (1). Traditionally, activation of heterotrimeric G proteins 1 is accomplished exclusively by the action of GPCRs, seven transmembrane-spanning proteins that typically reside in the plasma membrane. These receptors act as guanine nucleotide exchange factors (GEFs), binding the inactive GDP-bound conformation of G proteins and stimulating release of GDP from G␣. To ensure directionality of exchange, GEFs stabilize a nucleotide-free transition state of G␣ that is disrupted by binding of GTP (2, 3). This facilitates dissociation of G␣⅐GTP from the G␥ dimer and release of these proteins from the receptor. Dissociated G protein subunits then participate in interactions with a variety of effectors.G protein signaling is attenuated when G␣ hydrolyzes the ␥ phosphate of its bound GTP and G␣⅐GDP reassociates with ␥. GTPase-activating proteins (GAPs) facilitate the inactivation of many G proteins. Most of these GAPs contain a regulator of G protein signaling (RGS) domain that binds preferentially to the G␣⅐GTP transition state and accelerates GTPase activity (4, 5). More than 20 unique RGS domain-containing proteins have been discovered, and the nature of their G protein specificity and their mode of action in cells are subjects of intense interest (6, 7).Nonreceptor activators of G proteins may operate in lieu of or in conjunction with GPCRs to enhance signaling, but their physiological role is not well understood (8 -11). Activators of G protein signaling AGS1 and AGS3 were identified in a genetic screen in yeast designed to isolate expressed mammalian cDNAs that encode proteins that bypass the need for a receptor (12). AGS3 possesses G␣ guanine nucleotide dissociation inhibitor activity but may activate G proteins by liberating G␥ (10, 13). AGS1 encodes a Ras-like small GTPase that, when bound to GTP, possesses in vitro guanine nucleotide exchange activity for members of th...
Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the alpha subunit of Gq and Gi1. The effect on Gi1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both Gq- and Gi-dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.
Regulators of G protein signaling (RGS) proteins act as GTPase-activating proteins (GAPs) toward the ␣ subunits of heterotrimeric, signal-transducing G proteins. RGS11 contains a G protein ␥ subunit-like (GGL) domain between its Dishevelled͞Egl-10͞Pleckstrin and RGS domains. GGL domains are also found in RGS6, RGS7, RGS9, and the Caenorhabditis elegans protein EGL-10. Coexpression of RGS11 with different G  subunits reveals specific interaction between RGS11 and G 5 . The expression of mRNA for RGS11 and G 5 in human tissues overlaps. The G 5 ͞RGS11 heterodimer acts as a GAP on G ␣o , apparently selectively. RGS proteins that contain GGL domains appear to act as GAPs for G ␣ proteins and form complexes with specific G  subunits, adding to the combinatorial complexity of G protein-mediated signaling pathways.Proteins belonging to the RGS (regulators of G protein signaling) family constitute a newly appreciated group of at least 20 mammalian gene products that act as GTPaseactivating proteins (GAPs) on the ␣ subunits of heterotrimeric, signal-transducing G proteins (1-3). As such, RGS proteins can serve as negative regulators of G proteinmediated signaling pathways by speeding the inactivation of GTP-bound G ␣ subunits. Although several members of the RGS family are relatively simple Ϸ25 kDa proteins that contain little more than a characteristic RGS domain, others include modules that impart additional functions. For example, RGS12 can associate in vitro with certain G protein-coupled receptors by virtue of an alternatively spliced PDZ (PSD-95͞ Dlg͞Z0-1) domain (4), and p115, a guanine nucleotide exchange factor for the low-molecular-weight GTPase rho, contains an RGS domain that imparts sensitivity to regulation by G protein ␣ subunits (5, 6).We describe here a novel G protein ␥ subunit-like domain (GGL; pronounced giggle) that is found in several mammalian RGS proteins (RGS6, RGS7, RGS9, and RGS11) and in EGL-10, an RGS protein of Caenorhabditis elegans. The GGL domains of RGS11 and RGS7 interact preferentially with the G protein  5 subunit, and the complex of RGS11 and  5 has GAP activity toward the G protein ␣ o subunit. MATERIALS AND METHODSGeneration of Expression Constructs. cDNAs for RGS11 and various G protein subunits were cloned from human brain or retinal mRNA, from mouse retinal mRNA, or were obtained as described (7,8); all amplified cDNAs were verified by sequencing. Human RGS7 cDNA was a kind gift of Paul F. Worley (Johns Hopkins University). cDNAs encoding G protein subunits were subcloned into the mammalian expression vector pcDNA3.1-Zeo (Invitrogen), and G ␥ and RGS protein cDNAs were subcloned in-frame with an N-terminal tandem hemagglutinin (HA)-epitope tag into a modified pcDNA3.1 vector. Recombinant baculoviruses expressing native or hexahistidine-tagged RGS11 or G 5 subunits were generated by using the Bac-To-Bac system by following the manufacturer's protocols (Life Technologies, Gaithersburg, MD).In Vitro Transcription and Translation. Reactions were performed using the T...
Regulator of G-protein signaling (RGS) proteins increase the intrinsic guanosine triphosphatase (GTPase) activity of G-protein ␣ subunits in vitro, but how specific G-protein-coupled receptor systems are targeted for down-regulation by RGS proteins remains uncharacterized. Here, we describe the GTPase specificity of RGS12 and identify four alternatively spliced forms of human RGS12 mRNA. Two RGS12 isoforms of 6.3 and 5.7 kilobases (kb), encoding both an N-terminal PDZ (PSD-95/ Dlg/ZO-1) domain and the RGS domain, are expressed in most tissues, with highest levels observed in testis, ovary, spleen, cerebellum, and caudate nucleus. The 5.7-kb isoform has an alternative 3 end encoding a putative C-terminal PDZ domain docking site. Two smaller isoforms, of 3.1 and 3.7 kb, which lack the PDZ domain and encode the RGS domain with and without the alternative 3 end, respectively, are most abundantly expressed in brain, kidney, thymus, and prostate. In vitro biochemical assays indicate that RGS12 is a GTPaseactivating protein for G i class ␣ subunits. Biochemical and interaction trap experiments suggest that the RGS12 N terminus acts as a classical PDZ domain, binding selectively to C-terminal (A/S)-T-X-(L/V) motifs as found within both the interleukin-8 receptor B (CXCR2) and the alternative 3 exon form of RGS12. The presence of an alternatively spliced PDZ domain within RGS12 suggests a mechanism by which RGS proteins may target specific G-protein-coupled receptor systems for desensitization.The mammalian "regulators of G-protein signaling" (RGS) 1 gene family was first identified by sequence and functional similarity to fungal and nematode genes captured in genetic screens for negative regulators of specific G-protein-coupled receptor (GPCR) signals (1-3). In vitro biochemical analyses soon established that this gene family encodes potent accelerators ("GAPs") of the intrinsic GTP hydrolysis activity of Gprotein ␣ subunits, revealing a molecular mechanism by which RGS proteins drive G-proteins into their inactive GDP-bound form and hence down-regulate GPCR signal transduction in vivo (reviewed in Refs. 4 and 5). However, the mechanisms by which individual RGS proteins desensitize pathways activated by particular GPCRs remain to be elucidated. Tightly regulated transcription has been described for RGS1 (3), RGS2 (6), and RGS3-RGS11 (7), and palmitoylation of the cysteine-rich N terminus of G␣-interacting protein (GAIP) has also been observed (8); however, transcriptional regulation and post-translational modifications of particular RGS family members can each only be expected to afford a gross level of intracellular control over the temporal and spatial expression of G␣-directed GAP activity.We and others have hypothesized that regions outside the RGS fold contribute to regulation of G␣ GAP activity and/or targeting of individual RGS proteins to particular receptor signaling pathways (4, 5, 9, 10). Here, we report the GAP activity of RGS12 and identify a PDZ-like N-terminal sequence within two splice forms. PDZ domains...
Regulator of G-protein signaling (RGS) proteins areGTPase activating proteins (GAPs) of heterotrimeric Gproteins that alter the amplitude and kinetics of receptor-promoted signaling. In this study we defined the G-protein ␣-subunit selectivity of purified Sf9 cell-derived R7 proteins, a subfamily of RGS proteins (RGS6, -7, -9, and -11) containing a G␥-like (GGL) domain that mediates dimeric interaction with G 5 . G 5 /R7 dimers stimulated steady state GTPase activity of G␣-subunits of the G i family, but not of G␣ q or G␣ 11 , when added to proteoliposomes containing M2 or M1 muscarinic receptor-coupled G-protein heterotrimers. Concentration effect curves of the G 5 /R7 proteins revealed differences in potencies and efficacies toward G␣-subunits of the G i family. Although all four G 5 /R7 proteins exhibited similar potencies toward G␣ o , G 5 /RGS9 and G 5 /RGS11 were more potent GAPs of G␣ i1 , G␣ i2 , and G␣ i3 than were G 5 /RGS6 and G 5 /RGS7. The maximal GAP activity exhibited by G 5 /RGS11 was 2-to 4-fold higher than that of G 5 /RGS7 and G 5 /RGS9, with G 5 /RGS6 exhibiting an intermediate maximal GAP activity. Moreover, the less efficacious G 5 /RGS7 and G 5 /RGS9 inhibited G 5 / RGS11-stimulated GTPase activity of G␣ o . Therefore, R7 family RGS proteins are G i family-selective GAPs with potentially important differences in activities.Heterotrimeric guanine nucleotide-binding proteins (G-proteins) act as molecular switches in multiple GPCR 1 signaling pathways via regulation of specific effector molecules such as phospholipase C and adenylyl cyclase. The biological activity of G-protein ␣-subunits is determined by the identity of the bound guanine nucleotide (GTP or GDP), which in turn is governed by the relative rates of guanine nucleotide exchange and hydrolysis of GTP by the intrinsic GTPase activity of G␣-subunits.These opposing reactions are stimulated by agonist-occupied GPCR and GTPase-activating proteins (GAPs).Although some effector proteins exhibit GAP activity (1-3), the primary regulators of GTPase activity of G␣-subunits are a diverse family of regulator of G-protein signaling (RGS) proteins that act as GAPs for heterotrimeric G-protein ␣-subunits (4 -7). This family is defined by a conserved RGS domain, which markedly increases the rate of GTP hydrolysis by G␣-subunits and terminates effector activation by both G␣-and G␥-subunits. More than 30 RGS proteins have been identified and organized into subfamilies based on sequence similarity and domain structure. These families vary in size and complexity, from the R4 family whose structure is largely limited to the RGS domain to the R12 and RhoGEF families whose members are large multifunctional proteins containing several domains (for reviews see Refs. 8 -10).The R7 RGS family is a unique multidomain family, which consists of RGS proteins containing a novel G-␥-like (GGL) domain homologous to the G␥-subunit of heterotrimeric Gproteins (11). This domain, found in the mammalian proteins RGS6, -7, -9, and -11 and the Caenorhabdi...
Heterotrimeric G protein signaling specificity has been attributed to select combinations of G␣, , and ␥ subunits, their interactions with other signaling proteins, and their localization in the cell. With few exceptions, the G protein subunit combinations that exist in vivo and the significance of these specific combinations are largely unknown. We have begun to approach these problems in HeLa cells by: 1) determining the concentrations of G␣ and G subunits; 2) examining receptor-dependent activities of two effector systems (adenylyl cyclase and phospholipase C); and 3) systematically silencing each of the G␣ and G subunits by using small interfering RNA while quantifying resultant changes in effector function and the concentrations of other relevant proteins in the network. HeLa cells express equimolar amounts of total G␣ and G subunits. The most prevalent G␣ proteins were one member of each G␣ subfamily (G␣ s , G␣ i3 , G␣ 11 , and G␣ 13 ). We substantially abrogated expression of most of the G␣ and G proteins expressed in these cells, singly and some in combinations. As expected, agonistdependent activation of adenylyl cyclase or phospholipase C was specifically eliminated following the silencing of G␣ s or G␣ q/11 , respectively. We also confirmed that G subunits are necessary for stable accumulation of G␣ proteins in vivo. G subunits demonstrated little isoform specificity for receptor-dependent modulation of effector activity. We observed compensatory changes in G protein accumulation following silencing of individual genes, as well as an apparent reciprocal relationship between the expression of certain G␣ q and G␣ i subfamily members. These findings provide a foundation for understanding the mechanisms that regulate the adaptability and remarkable resilience of G protein signaling networks.Signal-transducing heterotrimeric G proteins are associated with the inner face of the plasma membrane, positioned as middlemen for activation by membrane-spanning, heptahelical receptors, and regulation of a variety of intracellular effectors. Interactions among these proteins are controlled by agonist-induced changes of receptor conformation and nucleotide-driven conformational changes of the ␣ subunits of the G proteins (G␣). A ligand-bound receptor catalyzes the exchange of GDP for GTP on a cognate G␣, and as a result, the (at least partial) dissociation of G␣ from a complex of G and G␥ subunits. These activated subunits are then capable of modulating the functional properties of effector proteins (e.g. adenylyl cyclases and phospholipases). The intrinsic GTPase activity of G␣ serves as a molecular timer, returning the protein to the GDP-bound state and allowing reformation of the inactive heterotrimer.Much remains to be learned about the specificity of G protein signaling in vivo, the relative importance of isoforms of G protein subunits with apparently redundant functions, and the qualitative and quantitative significance of the fact that many hundreds of G protein heterotrimers can be assembled from th...
Regulators of G protein signaling (RGS proteins) constitute a family of newly appreciated components of G protein-mediated signal transduction. With few exceptions, most information available on mammalian RGS proteins was gained by transfection/overexpression or in vitro experiments, with relatively little known about the endogenous counterparts. Transfection studies, typically of tagged RGS proteins, have been conducted to overcome the low natural abundance of endogenous RGS proteins. Because transfection studies can lead to imprecise or erroneous conclusions, we have developed antibodies of high specificity and sensitivity to focus study on endogenous proteins. Expression of both RGS4 and RGS7 was detected in rat brain tissue and cultured PC12 and AtT-20 cells. Endogenous RGS4 presented as a single 27-28-kDa protein. By contrast, cultured cells transfected with a plasmid encoding RGS4 expressed two observable forms of the protein, apparently due to utilization of distinct sites of initiation of protein synthesis. Subcellular localization of endogenous RGS4 revealed predominant association with membrane fractions, rather than in cytosolic fractions, where most heterologously expressed RGS4 has been found. Endogenous levels of RGS7 exceeded RGS4 by 30 -40-fold, and studies of cultured cells revealed regulatory differences between the two proteins. We observed that RGS4 mRNA and protein were concomitantly augmented with increased cell density and decreased by exposure of PC12M cells to nerve growth factor, whereas RGS7 was unaffected. Endogenous RGS7 was relatively stable, whereas proteolysis of endogenous RGS4 was a strong determinant of its lower level expression and short halflife. Although we searched without finding evidence for regulation of RGS4 proteolysis, the possibility remains that alterations in the degradation of this protein could provide a means to promptly alter patterns of signal transduction.G proteins transduce signals across the plasma membrane by sequential interactions with cell surface receptors and appropriate second messenger-producing effectors (e.g. enzymes and ion channels). These interactions are modulated by nucleotide-driven conformational changes in the ␣ subunits of heterotrimeric G proteins (G␣).1 A ligand-bound receptor catalyzes the exchange of GDP for GTP on its cognate G␣ and the dissociation of G␣ from the complex of G protein  and ␥ subunits (G␥). These dissociated subunits are competent to modulate the activity of effectors. The duration of G protein-mediated responses are dependent on the intrinsic GTPase rate of G␣ and on extrinsic factors, such as regulators of G protein signaling (RGS proteins).RGS proteins serve to regulate G protein signaling by functioning as GTPase-activating proteins (GAPs). GAP activity can sharpen the termination of a signal upon removal of a stimulus, attenuate a signal either as a feedback inhibitor or in response to a second input, promote regulatory association of other proteins, or redirect signaling within a G protein signaling network ...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.