Hadamard multiplexing is a measurement strategy that yields best sensitivity improvements over scanning measurements for signal-independent detector noise. The presence of photon noise degrades the performance of Hadamard multiplexing because of the increase of photon noise by the superposition of multiple signals. I derive the reduction of the sensitivity gain of a Hadamard measurement and an upper limit for the gain of any cyclic multiplexing strategy in the presence of photon noise. This upper limit clearly exceeds the reduced Hadamard gain and can be achieved by multiplexing sequences that differ from Hadamard S sequences but also share some similarities with respect to their autocorrelation. Examples of such sequences are given. As the analysis shows, the presence of photon noise limits the gain of multiplexing strategies to a finite value, which depends on the ratio between photon noise and detector noise and cannot be exceeded by increasing the number of multiplexed channels. In addition, only switching multiplex schemes, which superpose either all the light or no light of individual channels, can achieve the upper limit of the gain.
The past years have seen increasing interest in nonlinear optical microscopic imaging approaches for the investigation of diseases due to the method's unique capabilities of deep tissue penetration, 3D sectioning and molecular contrast. Its application in clinical routine diagnostics, however, is hampered by large and costly equipment requiring trained staff and regular maintenance, hence it has not yet matured to a reliable tool for application in clinics. In this contribution implementing a novel compact fiber laser system into a tailored designed laser scanning microscope results in a small footprint easy to use multimodal imaging platform enabling simultaneously highly efficient generation and acquisition of second harmonic generation (SHG), two-photon excited fluorescence (TPEF) as well as coherent anti-Stokes Raman scattering (CARS) signals with optimized CARS contrast for lipid imaging for label-free investigation of tissue samples. The instrument combining a laser source and a microscope features a unique combination of the highest NIR transmission and a fourfold enlarged field of view suited for investigating large tissue specimens. Despite its small size and turnkey operation rendering daily alignment dispensable the system provides the highest flexibility, an imaging speed of 1 megapixel per second and diffraction limited spatial resolution. This is illustrated by imaging samples of squamous cell carcinoma of the head and neck (HNSCC) and an animal model of atherosclerosis allowing for a complete characterization of the tissue composition and morphology, i.e. the tissue's morphochemistry. Highly valuable information for clinical diagnostics, e.g. monitoring the disease progression at the cellular level with molecular specificity, can be retrieved. Future combination with microscopic probes for in vivo imaging or even implementation in endoscopes will allow for in vivo grading of HNSCC and characterization of plaque deposits towards the detection of high risk plaques.
reduction, described in [5]. Any moves of quasi-optical components, that are bulky, could bring unreliable results to the system.
INCOHERENT OPTICAL CORRELATOR FOR PACKET HEADER RECOGNITION
There are several applications known for years using laser speckles for optical characterization of human skin. Laser speckle contrast analysis (LASCA) for non-invasive monitoring of capillary blood flow is one of them. 1,2 Recent reports calling the technique laser speckle contrast imaging (LSCI) describe the detailed opportunities and limitations of this technique for non-invasive blood flow quantification, 3 for example, for wound healing research. 4 However, besides blood flow there is more in there using laser speckles. Surface roughness quantification is an application 5 recently found to be interesting for skin analysis as well 6 and the assessment of skin tumour perfusion. The laser speckle technique has already been used to assess the skin roughness and skin tumours. [7][8][9] The contactless optical and label-free method has decisive advantages, particularly in the medical environment. In addition to
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