Although carvacrol (CAR) is considered an alternative antimicrobial for use in food, few is known about the influence of food-related parameters on its inhibitory effects against pathogens. This study assessed the influence of different amounts of proteins, using beef extract (BE) as a protein-rich source, lipids (LIP), using sunflower oil as a LIP-rich source, and pH values or their interaction on the inhibitory effects of CAR against Salmonella Typhimurium PT4 (ST) and Escherichia coli O157:H7 (EC). The specific maximum growth rate (μmax) and lag phase duration (λ) of the test pathogens when exposed to CAR in media with different amounts of BE (4, 6, and 8 g/100 mL), LIP (3.75, 5, and 6.25 mL/100 mL), and pH values (5, 5.5, and 6) were determined. The viable counts of the tested pathogens in media that promoted the highest and lowest μmax in the presence of CAR were monitored during 24 h. The lowest μmax of ST and EC exposed to 2.4 μL/mL (-1.29 and -0.82 log CFU/mL/h, respectively) or 4.8 μL/mL CAR (-1.44 and -2.17 log CFU/mL/h, respectively) were observed in media with the highest LIP amount (6.25 mL/100 mL) and pH value (pH 6). For both SE and EC, the longest λ (> 2 h) was verified in media where these pathogens showed the lowest μmax. These data indicate that the concomitant increase in LIP amounts and pH values affected positively the CAR inhibitory effects against the target pathogens. CAR (2.4 or 4.8 μL/mL) failed to inhibit the increase in ST and EC counts in media where the highest μmax values were previously observed. On the contrary, CAR inhibited the increase of ST counts (final counts 5 log CFU/mL) and decreased the EC counts (final counts 3.5 log CFU/mL) in media where the lowest μmax values were observed. These results show that the inhibitory effects of CAR on ST and EC in food matrices could be affected as a function of the interaction of LIP amounts and pH values.

The plant growth-promoting rhizobacteria (PGPRBs) is an interesting way to promote increased vegetable production. Here, we aimed to isolate, identify, and characterize PGPRBs by using biochemical tests, sequencing of 16S ribossomal DNA, in vitro and screening for indoleacetic acid production. The isolates were identified through VITEK® 2 Compact equipment, which is an automated system. We identified microorganisms such as Alcaligenes faecalis sp. faecalis, Pseudomonas putida, Proteus vulgaris, Providencia rettgeri, Serratia marcescens e Myroides sp. by performing vitek 2 biochemical tests. The analysis of sequencing data for 16S ribossomal DNA of the isolated bacteria showed presence of A. faecalis, Myroides sp., P. putida, P. vulgaris, Providencia sp. and Serratia sp. The in vitro screening of all isolated bacteria showed production of indoleacetic acid under presence of tryptophan, highlighting that higher concentrations were produced by Providencia sp. and Myroides sp. The rhizobacteria studied here have shown the potential to be used in the development of new products for plant growth-promoting.

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