A molecular assay based on a rapid DNA extraction protocol and the EnviroAmp Legionella Kits was used to detect LegioneUla species in bronchoalveolar fluid specimens. All LegioneUla strains isolated from tap water in hospitals could be detected distinctly. Both sensitivity and specificity were tested. In a prospective study,
Severe infection of the knee joint by Pseudallescheria boydii in a 23-year-old female resulted in complete destruction of the cartilaginous surface. The possibility of entry of the fungus through mild abrasions of unperforated skin is discussed. The clinical appearance is compared with that of Scedosporium prolificans. Antimycotic miconazole-itraconazole treatment, applied during a period of 4 months, was successful; no side-effects were noted.
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