Shifts in rainfall patterns and increasing temperatures associated with climate change are likely to cause widespread forest decline in regions where droughts are predicted to increase in duration and severity. One primary cause of productivity loss and plant mortality during drought is hydraulic failure. Drought stress creates trapped gas emboli in the water transport system, which reduces the ability of plants to supply water to leaves for photosynthetic gas exchange and can ultimately result in desiccation and mortality. At present we lack a clear picture of how thresholds to hydraulic failure vary across a broad range of species and environments, despite many individual experiments. Here we draw together published and unpublished data on the vulnerability of the transport system to drought-induced embolism for a large number of woody species, with a view to examining the likely consequences of climate change for forest biomes. We show that 70% of 226 forest species from 81 sites worldwide operate with narrow hydraulic safety margins against injurious levels of drought stress and therefore potentially face long-term reductions in productivity and survival if temperature and aridity increase as predicted for many regions across the globe. Safety margins are largely independent of mean annual precipitation, showing that there is global convergence in the vulnerability of forests to drought, with all forest biomes equally vulnerable to hydraulic failure regardless of their current rainfall environment. These findings provide insight into why drought-induced forest decline is occurring not only in arid regions but also in wet forests not normally considered at drought risk
SummaryThe evolution of lignified xylem allowed for the efficient transport of water under tension, but also exposed the vascular network to the risk of gas emboli and the spread of gas between xylem conduits, thus impeding sap transport to the leaves. A well-known hypothesis proposes that the safety of xylem (its ability to resist embolism formation and spread) should trade off against xylem efficiency (its capacity to transport water).We tested this safety-efficiency hypothesis in branch xylem across 335 angiosperm and 89 gymnosperm species. Safety was considered at three levels: the xylem water potentials where 12%, 50% and 88% of maximal conductivity are lost.Although correlations between safety and efficiency were weak (r 2 < 0.086), no species had high efficiency and high safety, supporting the idea for a safety-efficiency tradeoff. However, many species had low efficiency and low safety. Species with low efficiency and low safety were weakly associated (r 2 < 0.02 in most cases) with higher wood density, lower leaf-to sapwood-area and shorter stature. There appears to be no persuasive explanation for the considerable number of species with both low efficiency and low safety. These species represent a real challenge for understanding the evolution of xylem.
Recovery of hydraulic conductivity after the induction of embolisms was studied in woody stems of laurel (Laurus nobilis). Previous experiments confirming the recovery of hydraulic conductivity when xylem pressure potential was less than ؊1 MPa were repeated, and new experiments were done to investigate the changes in solute composition in xylem vessels during refilling. Xylem sap collected by perfusion of excised stem segments showed elevated levels of several ions during refilling. Stem segments were frozen in liquid N 2 to view refilling vessels using cryoscanning electron microscopy. Vessels could be found in all three states of presumed refilling: (a) mostly water with a little air, (b) mostly air with a little water, or (c) water droplets extruding from vessel pits adjacent to living cells. Radiographic probe microanalysis of refilling vessels revealed nondetectable levels of dissolved solutes. Results are discussed in terms of proposed mechanisms of refilling in vessels while surrounding vessels were at a xylem pressure potential of less than ؊1 MPa. We have concluded that none of the existing paradigms explains the results.Over the past two decades, scientists have found substantial evidence that the vulnerability of xylem to cavitation is an important factor in the adaptation of plants to the environment (Tyree and Sperry, 1989;Cochard et al., 1992;Salleo and Lo Gullo, 1993). The cavitation (droughtinduced embolism) of xylem has been detected in stems (Cochard and Tyree, 1990), leaves (Kikuta et al., 1997), and roots (Mencuccini and Comstock, 1997) and has appeared to limit effectively the possible distribution areas of plant species (Cochard et al., 1992). For example, the vulnerability of Holm oak to xylem embolism caused by both drought and freeze stress (Lo Gullo and Salleo, 1993) provides a convincing explanation for the distribution versus elevation and latitude of this species (Pignatti, 1982) in the Mediterranean region.The threshold xylem pressure for cavitation is close to the typical midday xylem pressure of many species in the field (Kikuta et al., 1997). Such a narrow safety margin (Sperry, 1995) is intrinsically dangerous for plant survival under adverse environmental conditions but might be of some advantage in buffering leaf water status (Dixon et al., 1984;Salleo et al., 1997) and in inducing stomatal closure (Sperry, 1995).Debate still exists about the possible mechanisms involved in xylem refilling after cavitation events induced by drought (Tyree and Cochard, 1996) and freezing (Sperry, 1995) stress. The existing paradigm suggests that embolism removal must occur by gas dissolution in the surrounding water. Henry's law states that the solubility of a gas in water is proportional to the partial pressure of the gas species adjacent to the water. Since water in plants is saturated with air at atmospheric pressure, the paradigm requires that the embolism be above atmospheric pressure for the gases to dissolve. Some experiments on angiosperms and gymnosperms fit this paradigm Yang and Tyr...
Xylem recovery from embolism was studied in Laurus nobilis L. stems that were induced to cavitate by combining negative xylem pressure potentials ( P X = = = = ----1.1 MPa) with positive air pressures ( P C ) applied using a pressure collar. Xylem refilling was measured by recording the percentage loss of hydraulic conductance (PLC) with respect to the maximum 2 min, 20 min and 15 h after pressure release. Sodium orthovanadate (an inhibitor of many ATP-ases) strongly inhibited xylem refilling while fusicoccin (a stimulator of the plasma membrane H + + + + -ATPase) promoted complete embolism reversal. So, the refilling process was interpreted to result from energy-dependent mechanisms. Stem girdling induced progressively larger inhibition to refilling the nearer to the embolized stem segment phloem was removed. The starch content of wood parenchyma was estimated as percentages of ray and vasicentric cells with high starch content with respect to the total, before and after stem embolism was induced. A closely linear positive relationship was found to exist between recovery from PLC and starch hydrolysis. This, was especially evident in vasicentric cells. A mechanism for xylem refilling based upon starch to sugar conversion and transport into embolized conduits, assisted by phloem pressure-driven radial mass flow is proposed.
We tested the hypothesis that hydraulic conductance per unit leaf surface area of plant shoots (K SL ) determines the maximum diurnal stomatal conductance (g L ) that can be reached by plants growing in the field. A second hypothesis was tested that some xylem cavitation cannot be avoided by transpiring plants and might act as a signal for regulating g L . Eleven woody species were studied, differing from each other with respect to taxonomy, wood anatomy and leaf habit. Maximum diurnal g L , transpiration rate (E L ), pre-dawn and minimum diurnal leaf water potential (Ψ pd and Ψ min , respectively) were measured in the field. The critical Ψ level at which stem cavitation was triggered (Ψ cav ) was measured on detached branches, using the acoustic method. A highpressure flow meter was used to measure maximum K SL of 1-year-old shoots. Both g L and E L were positively related to K SL . The whole-plant hydraulic conductance per unit leaf area (K WL ) of all the species studied, calculated as the ratio of E L to ∆Ψ (=Ψ pd -Ψ min ) was closely related to K SL . In every case, Ψ min (ranging between -0.85 and -1.35 MPa in the different species) dropped to the Ψ cav range or was <Ψ cav (ranging between -0.71 and -1.23 MPa), thus suggesting that some cavitationinduced embolism could not be avoided. The possibility is discussed that some cavitation-induced reduction in K SL is the signal for stomatal closure preventing runaway embolism. The lack of correlation of g L to Ψ cav is discussed in terms of the inconsistency of Ψ cav as an indicator of the vulnerability of plants to cavitation. No differences in hydraulic traits were observed between evergreen and deciduous species.
Water saving under drought stress is assured by stomatal closure driven by active (ABA-mediated) and/or passive (hydraulic-mediated) mechanisms. There is currently no comprehensive model nor any general consensus about the actual contribution and relative importance of each of the above factors in modulating stomatal closure in planta. In the present study, we assessed the contribution of passive (hydraulic) vs active (ABA mediated) mechanisms of stomatal closure in V. vinifera plants facing drought stress. Leaf gas exchange decreased progressively to zero during drought, and embolism-induced loss of hydraulic conductance in petioles peaked to ~50% in correspondence with strong daily limitation of stomatal conductance. Foliar ABA significantly increased only after complete stomatal closure had already occurred. Rewatering plants after complete stomatal closure and after foliar ABA reached maximum values did not induced stomatal re-opening, despite embolism recovery and water potential rise. Our data suggest that in grapevine stomatal conductance is primarily regulated by passive hydraulic mechanisms. Foliar ABA apparently limits leaf gas exchange over long-term, also preventing recovery of stomatal aperture upon rewatering, suggesting the occurrence of a mechanism of long-term down-regulation of transpiration to favor embolism repair and preserve water under conditions of fluctuating water availability and repeated drought events.
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