Aldoximes are known as floral and vegetative plant volatiles but also as biosynthetic intermediates for other plant defense compounds. While the cytochrome P450 monooxygenases (CYP) from the CYP79 family forming aldoximes as biosynthetic intermediates have been intensively studied, little is known about the enzymology of volatile aldoxime formation. We characterized two P450 enzymes, CYP79D6v3 and CYP79D7v2, which are involved in herbivore-induced aldoxime formation in western balsam poplar (Populus trichocarpa). Heterologous expression in Saccharomyces cerevisiae revealed that both enzymes produce a mixture of different aldoximes. Knockdown lines of CYP79D6/7 in gray poplar (Populus 3 canescens) exhibited a decreased emission of aldoximes, nitriles, and alcohols, emphasizing that the CYP79s catalyze the first step in the formation of a complex volatile blend. Aldoxime emission was found to be restricted to herbivore-damaged leaves and is closely correlated with CYP79D6 and CYP79D7 gene expression. The semi-volatile phenylacetaldoxime decreased survival and weight gain of gypsy moth (Lymantria dispar) caterpillars, suggesting that aldoximes may be involved in direct defense. The wide distribution of volatile aldoximes throughout the plant kingdom and the presence of CYP79 genes in all sequenced genomes of angiosperms suggest that volatile formation mediated by CYP79s is a general phenomenon in the plant kingdom.
SUMMARYNumerous plant species emit volatile nitriles upon herbivory, but the biosynthesis as well as the relevance of these nitrogenous compounds in plant-insect interactions remains unknown. Populus trichocarpa has been shown to produce a complex blend of nitrogenous volatiles, including aldoximes and nitriles, after herbivore attack. The aldoximes were previously reported to be derived from amino acids by the action of cytochrome P450 enzymes of the CYP79 family. Here we show that nitriles are derived from aldoximes by another type of P450 enzyme in P. trichocarpa. First, feeding of deuterium-labeled phenylacetaldoxime to poplar leaves resulted in incorporation of the label into benzyl cyanide, demonstrating that poplar volatile nitriles are derived from aldoximes. Then two P450 enzymes, CYP71B40v3 and CYP71B41v2, were characterized that produce aliphatic and aromatic nitriles from their respective aldoxime precursors. Both possess typical P450 sequence motifs but do not require added NADPH or cytochrome P450 reductase for catalysis. Since both enzymes are expressed after feeding by gypsy moth caterpillars, they are likely to be involved in herbivore-induced volatile nitrile emission in P. trichocarpa. Olfactometer experiments showed that these volatile nitriles have a strong repellent activity against gypsy moth caterpillars, suggesting they play a role in induced direct defense against poplar herbivores.
Plants emit complex mixtures of volatile organic compounds from floral and vegetative tissue, especially after herbivore damage, so it is difficult to associate individual compounds with activity towards pollinators, herbivores or herbivore enemies. Attention has usually focused upon the biological activity of the most abundant compounds; but here, we detail a number of reports implicating minor volatiles in attractant or deterrent roles. This is not surprising given the exquisite sensitivity of insect olfactory systems for certain substances. In this context, it is worth reconsidering the methods involved in sampling volatile compounds from plants, measuring their abundance and determining their biological activity to ensure that minor compounds are not overlooked. Here, we describe various experimental approaches and chemical and statistical methods that should increase the chance of detecting minor compounds with major biological activities.
BackgroundThe role of herbivore-induced plant volatiles as signals mediating the attraction of herbivore enemies is a well-known phenomenon. Studies with short-lived herbaceous plant species have shown that various biotic and abiotic factors can strongly affect the quantity, composition and timing of volatile emission dynamics. However, there is little knowledge on how these factors influence the volatile emission of long-lived woody perennials.The aim of this study was to investigate the temporal dynamics of herbivore-induced volatile emission of black poplar (Populus nigra) through several day-night cycles following the onset of herbivory. We also determined the influence of different herbivore species, caterpillars of the gypsy moth (Lymantria dispar) and poplar hawkmoth (Laothoe populi), and different herbivore developmental stages on emission.ResultsThe emission dynamics of major groups of volatile compounds differed strikingly in response to the timing of herbivory and the day-night cycle. The emission of aldoximes, salicyl aldehyde, and to a lesser extent, green leaf volatiles began shortly after herbivore attack and ceased quickly after herbivore removal, irrespective of the day-night cycle. However, the emission of most terpenes showed a more delayed reaction to the start and end of herbivory, and emission was significantly greater during the day compared to the night. The identity of the caterpillar species caused only slight changes in emission, but variation in developmental stage had a strong impact on volatile emission with early instar L. dispar inducing more nitrogenous volatiles and terpenoids than late instar caterpillars of the same species.ConclusionsThe results indicate that only a few of the many herbivore-induced black poplar volatiles are released in tight correlation with the timing of herbivory. These may represent the most reliable cues for herbivore enemies and, interestingly, have been shown in a recent study to be the best attractants for an herbivore enemy that parasitizes gypsy moth larvae feeding on black poplar.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0304-5) contains supplementary material, which is available to authorized users.
Herbivory is well known to trigger increased emission of volatile organic compounds (VOCs) from plants, but we know little about the responses of mature trees. We measured the volatiles emitted by leaves of old-growth black poplar ( Populus nigra ) trees after experimental damage by gypsy moth ( Lymantria dispar ) caterpillars in a floodplain forest, and studied the effect of herbivory on the transcript abundance of two genes involved in the biosynthesis of VOCs, and the accumulation of defence phytohormones. Herbivory significantly increased volatile emission from the experimentally damaged foliage, but not from adjacent undamaged leaves in the damaged branches (i.e., no systemic response). Methylbutyraldoximes, 4,8-dimethyl-1,3,7-nonatriene (DMNT), ( Z )-3-hexenol and ( E )-β-ocimene, amongst other compounds, were found to be important in distinguishing the blend of herbivore-damaged vs. undamaged leaves. Herbivory also increased expression of PnTPS3 (described here for the first time) and PnCYP79D6-v4 genes at the damaged sites, these genes encode for an ( E )-β-ocimene synthase and a P450 enzyme involved in aldoxime formation, respectively, demonstrating de novo biosynthesis of the volatiles produced. Herbivore-damaged leaves had significantly higher levels of jasmonic acid and its conjugate (−)-jasmonic acid-isoleucine. This study shows that mature trees in the field have a robust response to herbivory, producing induced volatiles at the damaged sites even after previous natural herbivory and under changing environmental conditions, however, further studies are needed to establish whether the observed absence of systemic responses is typical of mature poplar trees or if specific conditions are required for their induction.
The behavioral mechanisms of mating disruption in Guatemalan potato moth Tecia solanivora were studied using the sex pheromone components, (E)-3-dodecenyl acetate, (Z)-3-dodecenyl acetate, and dodecyl acetate, formulated in a 100:1:20-ratio mimicking the female-produced blend, and in a 100:56:100 off-blend ratio. The mode of action of these two blends was tested in mating disruption experiments in the field and in a greenhouse, as well as in a laboratory wind tunnel. Field treatments with both blends at 80 g pheromone per ha reduced male attraction to trap lures baited with 100 μg of female sex pheromone. In mesh-house treatments, these two blends were equally effective at reducing male attraction to traps baited with live females and mating of caged females. Subsequent flight tunnel tests corroborated that both blends reduced attraction of naive males to calling females, and pre-exposure of males with either dispenser blend for 24 hr resulted in a strongly reduced response to calling females. The pre-exposure effect was reversible, with males again responsive after 24 hr in clean air. The two dispenser formulations produced a similar effect on male behavior, despite the differences in blend composition. One mating disruption dispenser formulated with either the female-blend or off-blend elicited the same rate of male upwind attraction in a wind-tunnel bioassay. Sensory overload and camouflage, therefore, are contributing mechanisms to mating disruption using either blend. The off-blend, which is more economical to synthesize, is a valuable tool for further development of mating disruption against this major pest of potatoes in Latin America.
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