No abstract

No abstract

No abstract

No abstract

In a circular economy strategy, waste resources can be used for the biological production of high added-value substances, such as medium chain fatty acids (MCFAs), thus minimising waste and favouring a sustainable process. This study investigates single-stage fermentation processes for the production of MCFAs in a semi-continuous reactor treating the extract of real food waste (FW), without the addition of external electron donors. Two sequential acidogenic fermentation tests were carried out at an organic loading rate (OLR) of 5 and 15 gCOD L−1d−1 with a hydraulic retention time of 4 days and pH controlled at 6 ± 0.2. The highest level of caproate (4.8 g L−1) was observed at OLR of 15 gCOD L−1d−1 with a microbiome mainly composed by lactate-producing Actinomyces, Atopobium, and Olsenella species and caproate-producing Pseudoramibacter. Metagenomic analysis revealed the presence of key enzymes for the production of lactate, such as lactate dehydrogenase and pyruvate ferredoxin oxidoreductase, as well as several enzymes involved in the reverse β-oxidation pathway, thus suggesting the occurrence of a lactate-based chain elongation process.

No abstract

The agricultural use of good quality sludge represents a value-added route to ensure growth sustainability in Europe, where raw material availability, for example, for phosphorus, is insufficient to meet demand. However, the possible presence of pathogens, pharmaceuticals and heavy metals requires specific regulations to minimize sludge-related health issues and environmental risks. The current regulation on sludge agricultural use applied by many EU countries is here presented and compared, highlighting scarce harmonization of the legislative framework among Member States. Actual issues, such as the fate of emerging micropollutants and microplastics in sludge-amended soils, and public health concerns regarding sludge spreading during the COVID-19 epidemic, are considered, too.

No abstract