A central challenge in microplastics (MP, diameter < 5 mm) research is the analysis of small plastic particles in an efficient manner. This review focuses on the recent application of infrared hyperspectral imaging (HSI) to analyze MP. We provide a narrative context for understanding technical principles of HSI followed by a systematic review and discussion of the variety of approaches to apply HSI to MP research, including instrumentation, data collection and analysis. HSI was successfully applied to analyze dry MP > 250 μm, with drastic improvements in analysis time as compared with the best available technology, such as Fourier transform infrared (FT-IR) and Raman spectroscopy. Primary challenges we identified through the review include improving spatial resolution to detect smaller MP and development of robust models for data analysis. Parameters and practices for reporting quality assurance and quality control measures are summarized and recommendations are made for future research. We conclude that HSI is a promising technology for MP analysis but requires adaptation for this new application.
Throughout the past decade, many studies have reported adverse effects in biota following microplastic exposure. Yet, the field is still emerging as the current understanding of microplastic toxicity is limited. At the same time, recent legislative mandates have required environmental regulators to devise strategies to mitigate microplastic pollution and develop health-based thresholds for the protection of human and ecosystem health. The current publication rate also presents a unique challenge as scientists, environmental managers, and other communities may find it difficult to keep up with microplastic research as it rapidly evolves. At present, there is no tool that compiles and synthesizes the data from these studies to allow for visualization, interpretation, or analysis. Here, we present the Toxicity of Microplastics Explorer (ToMEx), an open access database and open source accompanying R Shiny web application that enables users to upload, search, visualize, and analyze microplastic toxicity data. Though ToMEx was originally created to facilitate the development of health-based thresholds to support California legislations, maintaining the database by the greater scientific community will be invaluable to furthering research and informing policies globally. The database and web applications may be accessed at https://microplastics.sccwrp.org/.
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