Understanding the role played by sunken vessels in Mediterranean marine ecosystems is acquiring increasing importance. The aim of this research was to study the fish communities associated with four shipwrecks, by means of underwater visual censuses performed by a remotely operated vehicle, and to test the differences in composition of fish assemblages between these shipwrecks and the adjacent soft bottoms, considered as control sites. Multivariate analysis on the total fish assemblage showed significant differences between wrecks and controls. Results also showed higher levels of species richness and abundance near all wrecks than at a short distance from them on soft bottoms, thus indicating that these sunken vessels, thanks to their higher habitat complexity, act as artificial reefs, attracting aggregations of fish species and leading to a greater diversification of the local fish assemblage. Nevertheless, shipwrecks, which are an ideal target for recreational fishermen, could contribute to the over-exploitation of some high-value fish species, such asMycteroperca rubra, Dentex dentexandDiplodusspp., attracted by the artificial hard substrate of the vessel-reefs. The recent European directives suggest an urgent need for a better understanding of the crucial role played by these potential sources of pollutants on marine environments and ecosystems. An ecosystem approach to study and monitor these pollutant sources is, therefore, mandatory for appropriate remediation and/or mitigation of the potential negative effects on a productive and healthy ocean.

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We report the isolation and characterization of an unusual strain of Streptococcus salivarius, 3C30, displaying both the macrolide-lincosamide-streptogramin B and the tetracycline resistance phenotypes. It harbours the mef(E), erm(B), and tet(M) genes carried by different genetic elements. The genetic element carrying mef(E), named mega, was investigated by long PCR and sequencing, while the presence of the Tn3872-like element, carrying tet(M) and erm(B), was demonstrated by sequencing of both the int-xis-Tn and the fragment between the two resistance genes. In strain 3C30 the mega element is 5388 bp in size and its nucleotide sequence is identical to that of the element described previously in S. salivarius, with the exception of a 912 bp deletion at the left end. The composite Tn3872-like element appeared to be nonconjugative while the mega element was transferred by conjugation to Streptococcus pneumoniae. It was, however, impossible to transfer it again from these transconjugants to other strains. In addition, only in the 3C30 strain did mega form circular structures, as identified by real-time PCR. In conclusion, we found a clinical strain of S. salivarius carrying both mega and Tn3872-like genetic elements. Mega is transferable by conjugation to S. pneumoniae but it is not transferable again from the transconjugants, suggesting a possible mobilization by recombinases of the coresident Tn3872-like transposon.

The ongoing spread of electric sustainable mobility is transforming the local ways of transport in metropolitan areas. This is meant to be extended outside of big cities in the near future thanks to new technological developments. Little towns should adapt to these changes, as they are located geographically far from the big cities and are generally characterized by low economic and demographic indicators. Hence, little towns must keep pace with these changes in mobility to avoid being isolated from the main cities in a country. People living in the countryside usually move toward big cities for various reasons, either related to work or living necessities. Therefore, it must be possible to conduct usual displacements through the use of electric vehicles (EVs), i.e., reaching the destinations and supplying the batteries through charging infrastructures. This paper studies the full implementation of electric mobility applied in the case of Cuenca, a city located in middle Spain. A brief geographical context is provided, together with the routes and destinations of interest considered. Then, different EVs are considered and an analytical vehicle model is provided. The model was exploited to simulate the electrical energy demand to reach the destinations chosen; the results allow comparing the performances offered by different types of EVs. This aspect is then considered as the basis to propose further upgrades in the charging infrastructures where needed, to comply with the widespread use of electric mobility.

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