Etiology of acute undifferentiated febrile syndrome (AUFS) is often unknown, leading to inaccurate diagnosis and treatment. Villeta town has been identified as an endemic area for spotted fever group (SFG) rickettsioses but little is known about possible amplifier hosts and other species different from. Besides, few studies have approached other AUFS etiologies in the region. We investigated the role of dengue, leptospirosis, rickettsioses, human anaplasmosis, and Q fever as possible causes of AUFS in patients from Villeta. Sera specimens and ticks from animals as well as ticks from vegetation were studied for the presence of different spp. Among 104 sera from patients with AUFS, 16.4%, 24.0%, and 2.9% patients seroconverted to dengue,, and SFG , respectively, with a case of probable coinfection or cross-reaction with. None of the samples were reactive for . Sera samples from 74 horses, 118 dogs, and 62 bovines were collected and showed 33.8%, 14.4%, and 50.0% of seroprevalence for SFG, respectively. A total of 1,287 ixodid ticks were collected from animals/vegetation and processed in pools for polymerase chain reaction. Among them, 1.7% was positive for genes, and, , and spp. were found. These results confirm the circulation of dengue, different SFG species and the relevance of other etiologies like leptospirosis and human anaplasmosis. Further studies must identify different epidemiological variables to establish proper surveillance and control programs.
The difficulties in the management and use of biosolids in Colombia make it necessary to evaluate and analyze the factors involved through various methodologies to achieve the effective management and recycling of this type of waste. The objective of this study was to evaluate the management of sludge and biosolids from a WWTP in a Colombian municipality through the application of three methodologies (SWOT/TOWS, surveys, and stakeholder (player) weighing) for their subsequent use in agriculture. As a result, strategies were proposed at the regulatory, organizational policy, and entity integration levels, among others. It was identified that about 93.6% of the people surveyed had a positive attitude towards the use of biosolids in agriculture, despite recognizing the existence of a risk (27.3%) in this type of practice. On the other hand, regarding the communication of WWTP management of these wastes, they perceived that it to be absent (65%) and the lack of knowledge regarding the destination of these wastes was even greater (72.7%). Through the weighting of actors, 16 players were classified with whom it is necessary to work closely, regularly, or occasionally. The methodologies proposed will allow similar WWTPs to optimize their processes through continuous improvement and joint work between the different entities and communities. It is recommended that other methodologies be used to evaluate player position level in relation to planned strategies, as well as the level of associations of one player with another, independent of power and influence.
The reuse of biosolids in agriculture and its inclusion within the circular economy model requires evaluating and analyzing factors that intervene in its management. The objective of the study was to analyze those factors that influence the management and use of biosolids. Fifty-three actors were questioned, and their answers were analyzed using two prospective methods and Social Network Analysis (SNA) identifying between 14 and 19 variables. Six should be prioritized due to their criticality and potential in management and reuse scenarios. It was observed that the formulation of objectives, such as the improvement of infrastructure, creation of an institutional policy, and the establishment of definitions for the kinds of biosolids, are opposed by internal agents. Seven key actors and four to six determining agents were identified in the scenarios. The network of management and use of biosolids in agriculture presented low density (0.28) and the exclusive action of three key actors. Consequently, the participation of a greater number of better-connected actors is required to project networks with a higher density (between 0.49 and 0.57), facilitating the diffusion of information and the inclusion of new actors not previously contemplated. The application of prospective and SNA methodologies focused on biosolids allows the prioritization of determinants, the evaluation of the level of involvement and communication between actors, and other aspects that have not been considered previously in the management of WWTPs in Colombia.
The use of a single host strain that allows for an evaluation of the levels of total coliphages in any type of environmental sample would facilitate the detection of and reduction in complexity and costs, favoring countries or areas with technical and economic limitations. The CB390 strain is a candidate for this type of simultaneous determinations, mainly in water samples. The objective of the study was to establish the recovery capacity of the CB390 strain in solid and semi-solid samples and to evaluate the microbiological quality of the sludge generated and stabilized by lime and drying beds in two WWTPs in Colombia. The results of both matrices indicated that CB390 recovered similar numbers of total coliphages (p > 0.05) against the two host strains when evaluated separately. Only the drying bed treatment was able to reduce between 2.0 and 2.9 Log10 units for some microorganisms, while the addition of lime achieved a maximum reduction of 1.3 Log10 units for E. coli. In conclusion, the CB390 strain can be used in solid and semi-solid samples, and the treatment in a drying bed provided a product of microbiological quality. However, the results are influenced by the infrastructure of the WWTP, the treatment conditions, and the monitoring of the stabilization processes.
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