Introduction: Oral Squamous Cell Carcinoma (OSCC) represents approximately 96% of the entire oral cancers. Epithelial-mesenchymal transition (EMT) is a factor contributing to the poor prognosis associated with OSCC. α-mangostin is one of the xanthones which show anti-cancer activities against some types of cancers and can suppress EMT-induced invasion by increasing E-cadherin expression. This study aimed to identify the viability of α-Mangostin to reduce the viable cells of HOC313. Methods: The role of α-mangostin to induce HOC313 cell culture at various concentrations which conducted on two groups: control group using only HOC313 cell line and intervention group comprising HOC313 cell line which added various concentrations. In this present study, cells were treated after reaching the confluency level of 80% in 5x103 cells/well. α-mangostin used had six concentrations: 1.25 µM, 2.5 µM, 3.75 µM, 5 µM, 6.25 µM, and 7.5 µM. Results: Concentration of α-mangostin had a significant effect on cell viability, p-value obtained was at 0.023 (p < 0.05). The Mann-Whitney test was also performed to identify significant differences in cell viability between control cells and all treatment cells were at 2.5 mg/ml and 5 mg/ml with the value p = 0.02 (p < 0.05). The concentrations α-mangostin at 1.25, 2.5, 3.75, 5, 6.25, and 7.5 µM is unable to reduce cell viability of HOC313. Conclusion: Low α-mangostin concentrations possibly result in a biphasic effect which leads to increase the viability cell of HOC313 cell line. Therefore, high α-mangostin concentrations might effectively inhibit cancer cell growth, migration, and invasion.
Background: Loss of permanent teeth after tooth extraction without replacement of missing teeth can result in impaired masticatory, esthetic, phonetic functions, and impaired balance of the masticatory organ in the mouth. Therefore, a method is needed to inhibit the alveolar bone resorption process so that the dimensions of the tooth socket can be maintained vertically or horizontally until the time of implant placement, which is called the socket preservation procedure. α-mangostin is known to have a potential anti-inflammatory effect and most likely can be used as a potential therapeutic agent to inhibit bone resorption caused by posttooth extraction inflammatory processes. Aims: The aim of the study was to determine the effect on the inflammatory process and osteogenesis on osteoblast cell line culture by induction with lipopolysaccharide (LPS) and α-mangostin. Materials and Methods: This was an in vitro laboratory experimental study on mouse osteoblast cell line culture. The treatment was given with LPS, α-mangostin, and combination on osteogenic medium, using the same concentration for all concentrates. The sample will then be processed and analyzed using the real-time polymerase chain reaction. Results: The highest interleukin-11 (IL-11) gene expression was found in α-mangostin treatment, but there was no significant difference in IL-11 expression between the study groups. The highest runt-related transcription factor-2 (RUNX-2) gene expression was found in a group that received induction with LPS and α-mangostin, and from these results, it was found that there was a significant difference in RUNX-2 expression between the study groups. Conclusions: LPS and α-mangostin can increase osteogenesis in osteoblast cell culture in the osteogenic medium.
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