Saccharin has been used as a nonnutritive sweetenjlng agent for nearly a century. Studies conducted over 50 years ago indicated that it is completely, or nearly completely, absorbed from the gastrointestinal tract and rapidly excreted in the urine in unchanged form ( 1). Largely ignored since these early studies, several factors make a reassessment of saccharin )metabolism advisable: (i) evidence of increasing saccharin consumption, estimated at more than 5 million pounds in the United States in 1970, (ii) a heightened awareness of possible adverse effects of many food additives, and (iii) the paucity of metabolic data based on modern methods, of investigation.We report below a study of absorption, distribution, metabolism, and excretion of saccharin in rhesus monkeys.Methods. Eight adult female rhesus monkeys (Macaca mulntta) , weighing from 5.7 to 6.7 kg, were utilized in the study. After an overnight fast, the animals were tranquilized with phencyclidine hydrochloride (Sernylan, Parke Davis and Co., Detroit, Mich.) 1 mg/kg intramuscularly. With the animal in the recumbent position, an incision was made in the groin, exposing the femoral vessels. A polyethylene catheter was inserted through the femoral vein into the inferior vena cava for sequential blood sampling. The incisions were then sutured and the animal was restrained in the dorsal recumbent position without use of additional phencyclidine. An indwdimg catheter was placed in the urinary
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