An improved incubator for the determination of the photosynthesis rate‐irradiance curve is described. It allows measurements of carbon fixation in waters with low chlorophyll a concentration by using rather large seawater volume. The irradiance gradient inside the incubation chambers is reproducible and can be accurately determined. With the capability of simultaneous measurements on 10 water samples, extensive determination of vertical profiles of photosynthetic parameters is achievable. Examples of photosynthetic parameters are given for waters collected in eutrophic, mesotrophic, and oligotrophic sites of the tropical Atlantic Ocean.
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