This study aimed to evaluate the behavior of Mentha piperita under Cd, Pb, Ni, and As soil contamination and their transfer from soil in plants as well as translocation in the roots/stems/leaves system compared with a control without metal addition. The mint seedlings were exposed for a three-month period using two metal mixtures in the same concentrations such as AsCd and AsCdNiPb (23.7 mg/kg As, 5 mg/kg Cd, 136 mg/kg Ni, and 95 mg/kg Pb). The results of metal concentration in plants showed that Cd, Ni, and Pb were accumulated in different parts of the plant, except for As. In plants organs, the order of metal accumulation was roots > stems > leaves. No significant impact on the growth, development, and chlorophyll content compared to the control was observed in the first month of exposure. After three months of exposure, phytotoxic effects occurred. Generally, the transfer coefficients and translocation factors values were less than 1, indicating that Mentha piperita immobilized the metals in root. The laboratory experiments highlighted that for a short period of time, Mentha piperita has the capacity to stabilize the metals at the root level and was a metal-tolerant plant when using a garden rich-substrate.
The paper presents a monitoring study of the platinum group metals concentrations in 17 samples of road dust and vegetation from areas with heavy car traffic on several European and national roads in eastern Romania. The Ir, Pd, Pt, Rh, Ru concentrations were determined by the ICP-MS technique, the reported values being below the method�s limit of determination for Ir, Pt and Ru both in soil and vegetation. As regards the Pd content, it showed a maximum of 794 mg / kg dry matter in Focsani area (DN2), an area where, according to national data published by National Road Infrastructure Management Company, the traffic average is 16,000 vehicles / 24 h. Approximately 50% of the collected vegetation samples showed a Pd transfer factor from the solid part to the vegetation higher than 0.5, thus indicating the existence of Pd toxic compounds bioavailable for vegetation. The highest concentrations of Rh in soil were recorded on a high traffic section on the E85, in Ramnicu Sarat area, where the traffic interval ranges from 8,001 to 16,000 vehicles / 24 h.
Collagen is one of the predominant proteins found in all multicellular animals. In this paper, the performance of the ultrafiltration process (UF) for concentrating the collagen protein was studied by performing batch experiments using a membrane module with an effective area of 28 cm 2 and equipped with a regenerated cellulose membrane with a molecular weight cutoff of 5 kDa. The feed solution consisted of aqueous solutions of bovine collagen hydrolysate with a molecular weight of 10 kDa. The effects of feed concentration (1%, 2%, 3%, 4%, and 5%) on the performance were determined. The optimum operating conditions were: a transmembrane pressure of 5 bar and a temperature of 25 C. The results showed that the permeability decreases with the increase of the volumetric concentration factor (VCF) for all five concentrated solutions of collagen tested. Also, the rejection of proteins increases with the increase in the concentration of the collagen solutions. The study confirms that the clogging phenomenon was dependent on the increase of concentration. Further, a mathematical modeling of fouling mechanism was analyzed based on Hermia's model. The concentration of the collagen solutions was also highlighted by viscosity measurements.
The aim of the study was to assess the groundwater quality in a rural area affected by the abandoned pyrite ash waste dumps. The abundance of major ions in groundwater depends largely on the nature of the rocks, climatic conditions, and mobility. To evaluate geochemical processes, 30 groundwater samples collected from Valea Calugareasca, Prahova County, Romania, were analyzed for the major anions (NO3−, SO42−, Cl−, HCO3−, and F−) and cations (Ca2+, Mg2+, Na+, and K+), which are naturally highly variable due to climatic and geographical location conditions. Ca2+, Na+, Mg2+, and K+ varied between 118 and 275 mg/L, 32 and 160 mg/L, 12.2 and 78.4 mg/L, and 0.21 and 4.48 mg/L, respectively. NO3− levels exceeding the World Health Organization (WHO) limit of 50 mg/L were identified in 17% of the groundwater samples, mainly as result of fertilizers applied to agricultural activities. The hydrogeochemical study identified dolomite dissolution and halite precipitation as natural sources of ions as well as the presence of pyrite as a source of SO42− ions in 60% of the samples. The sulfate content varied between 125 and 262 mg/L. Bicarbonate and chloride concentrations varied between 202 and 530 mg/L and 21 and 212 mg/L. The saturation index indicates the contribution of Ca2+ ions in the groundwater samples came from some processes of dissolving rocks such as aragonites (values between 1.27 and 2.69) and calcites (values between 1.43 and 2.82). Negative halite values indicated that salt accumulation results from precipitation processes. Only 10% of the analyzed groundwater samples were suitable for human consumption, the samples being situated on the hill, far away from the pyrite ash waste dumps and agricultural land.
A study for evaluating the quality of wastewater discharged into the sewerage network of Bucharest, for several economic agents with various activity profiles: a car wash, a sweet producer, and a provider of automatic access systems were presented in this paper. The study was conducted over five years (2013- 2017). The results obtained for the analyzed parameters were compared with the maximum allowed values (MAV) by the legislation in force. The results showed that for the car wash there was only one exceeding of the chemical oxygen demand (COD) parameter during the whole study period. The sweet producer and the provider of the automatic access systems evacuated wastewater with exceedances of MAV for the parameters: COD, BOD5, zinc, suspended solids, extractable substances in organic solvents, and total phosphorus. To evaluate the quality of the wastewater discharged by these two agents, the quality indices of wastewater (WWQI) were calculated. For the sweet producer, the calculated water quality indices had values between 62.4 - 92.7%, with a classification of wastewater, discharged in the quality class: marginal to good quality. For the provider of automatic access systems, the value of the quality index was between 74.2 and 85.5, the discharged wastewater being considered fair or good.
Medicinal plants are, for a considerable part of the population, an important source of treatment for certain diseases. They contain essential nutrients for the human body such as magnesium, iron , and zinc. The present study shows the influence of the addition of As, Cd, Ni, Pb on mineral nutrients in different organs of Mentha piperita. The experiments were carried out in the laboratory for a period of three months (May-July). Mentha piperita plants were AsCd and AsCdNiPb exposed up to final concentrations corresponding to the soil intervention threshold according to Romanian Order no.756/1997 (25 mg/kg As, 5 mg/kg Cd, 150 mg/kg Ni and 100 mg/ kg Pb). Simultaneously with these experiments, a control experiment (M) was performed. To evaluate the effect of the addition of AsCd and AsCdNiPb on the accumulation and transfer of Ca, Cr, Cu, Mn, Mo, Fe and Zn, the transfer coefficient (TC), the translocation factor (TF), and the enrichment factor (EF) were calculated. A higher concentration of Ca, Cr, Cu, Fe, Mn, Mo, and Zn was observed especially in the mint root in the experiments in which AsCdNiPb was added compared to those in which only AsCd was added. The AsCdNiPb addition did not influence the translocation of micro and macronutrients from the root to the aerial (edible) parts of the plant. In the case of the AsCd, addition, the translocation of zinc from the root to the aerial parts (leaves and stem) of the plant was increased.
As groundwater is used as a source of drinking water, monitoring its quality is essential due to possible adverse effects on human health. Nitrogen compounds (nitrates, nitrites, ammonia) within certain concentration limits are natural components of the nitrogen cycle. Due to anthropogenic activities, high concentrations of nitrogen compounds are released into groundwater. The aim of this study was to evaluate the chemistry of groundwater in a suburban area in northeastern Romania, the sources of pollution with nitrogen compounds (nitrates, nitrites, ammonium), and the non-carcinogenic risk to human health associated with consumption in different groups age (women, men, and children) in the investigated region. The results showed that the concentration of nitrogen compounds varies from 5.12 to 98.3 mg/L for nitrates, from 0.008 to 85.2 mg/L for ammonium, and from 0.001 to 1.12 mg/L for nitrites. The maximum admissible concentrations have been exceeding 25%, 40% and respectively 10% of the total analyzed samples Bivariate graphs and Principal Component Analysis (PCA) were used to identify potential sources of nitrate, nitrites, and ammonium pollution of groundwater in the study area. The non-carcinogenic risk assessment for water consumption showed a hazard index (HItotal) for nitrogen compounds in groundwater in the investigated region, which ranged from 0.037 to 2.856 for men, between 0.054 and 3.427 for women, respectively between 0.080 and 6.145, for children. Spatial distribution maps using the Inverse Distance Weighting technique presented the geographical areas with the probability of groundwater contamination with nitrate, nitrite, and ammonium and the areas that pose a risk to human health by consuming groundwater in the study area for the three groups: men, women and children.
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