The effect of extraction solvents and temperatures on extraction yields
of monosaccharides, sucrose,
and raffinose oligosaccharides from plant materials was investigated.
Toasted soybean meal, cotton
seed meal, field peas, and a feed mixture were extracted in either
water, 50% (v/v), or 80% (v/v)
aqueous methanol or ethanol at 20 or 50 °C or at the boiling point of
the solvent. Extraction in
80% (v/v) alcohol was strongly influenced by the extraction
temperature and maximum extraction
was only achieved at the boiling point. Extraction in water and
50% (v/v) methanol or ethanol was
less heat sensitive and gave comparable results. Aqueous ethanol
(50%, v/v) was as effective as
50% (v/v) methanol, whereas lower yields were seen at higher alcohol
strength. There was no
consistent difference in the extraction yield when comparing reflux
with constant stirring and water
bath with occasional mixing for any of the extraction solvents
used.
Keywords: Oligosaccharides; α-galactosides; HPLC; extraction solvent;
extraction temperature
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