The absorption and fluorescence spectra and second harmonic generation (SHG) of the insoluble monolayer
of bis-(N-ethyl,N-octadecyl)rhodamine (RhC18) at the air−water interface have been measured. These
spectra were affected significantly by compression, and the observed changes were ascribed to the formation
and structural rearrangement of aggregated species on the water surface during compression. The
spectroscopic behavior of the monolayer was explained in accordance with its rheological properties, and
the transition from disordered monomers to dimers, from dimers to aggregates, and from aggregates to
two-dimensional arrays was proposed. SHG studies revealed that the RhC18 molecules in the expanded
film region are oriented with their C
2-axis tilted away from the surface normal on angle θ distributed in
the range of 31−39°. The rotational distribution around the C
2-axis was assumed to be 45−60° according
to preferable intermolecular interactions with the water subphase and surrounding molecules. The θ angle
distribution became slightly narrow because of the increase of molecular ordering caused by two-dimensional
external pressure. The sharp increase of SHG intensity and the phase shift observed at high compression
were ascribed to the formation of blue-shifted aggregates with their electronic transition being in resonance
with the incident laser frequency. The results of spectroscopic and SHG studies were jointly analyzed, and
the structural rearrangement within the monolayer during compression was described.
Ultrafast change in refractive index after excitation by a femtosecond laser pulse was observed for dilute Auramine O solutions in low-viscosity solvents by detecting the change as an ultrafast lensing effect (ULE). The decay of the ULE signals was found to consist of two components, and both their relaxation times depended on solvents. This is the first reported observation of the faster component by this technique. The slower component had a similar relaxation time to that of the fluorescence lifetime. Molecular orbital calculation results attributed the faster to relaxation in the lowest excited singlet state S 1 by way of internal rotation and the slower to the subsequent relaxation from S 1 to the ground state. Solvent dependence of the experimentally determined relaxation time was strongly correlated with the molecular weight of the solvents rather than their bulk viscosity. A simplified model explaining these results was proposed in which solute and solvent molecules interact only via hydrogen bonding, the strength of which is solvent independent.
A new method has been proposed to determine the equilibrium between the bulk and the surface by directly
measuring surface concentrations using laser two-photon ionization. This method has been applied to
pyrenebutyric acid. The surface concentrations depended on the pH of the solution and were analyzed on the
basis of two equilibrium constants and two distribution coefficients. Most pyrenebutyric acid stays on the
surface at pH = 2.2. The equilibrium constant, pK
a, of pyrenebutyric acid on the water surface was determined
to be 7.85 ± 0.13, and this value is shifted to higher value than that in the bulk (4.76). The distribution
coefficient of the neutral pyrenebutyric acid was determined as (5.9 ± 2.8) × 10-2 m, and that of pyrenebutyric
anion as (4.82 ± 0.1) × 10-5 m. The ratio of the distribution coefficient of the neutral pyrenebutyric acid to
that of pyrenebutyric anion was determined to be (1.2 ± 0.6) × 103. These findings indicate that the equilibrium
shifts toward the neutral form on the water surface. Laser two-photon ionization was found to be a sensitive
and powerful technique to analyze equilibrium on the surface and that between the surface and the bulk.
Orientational arrangement of long-chain Fluorescein O322 molecules within the monolayer at the air/water interface induced by the compression process was studied by surface second harmonic generation.
Compression does not change the average tilt angle of the chromophore in any significant way, but it
induces the alignment of the chromophore along a preferred direction perpendicular to the compression
direction, originating the in-plane optical anisotropy. A model has been proposed on the orientational
ordering of Fluorescein O322 molecules within the monolayer induced by the compression process.
Pressure-induced structure ordering of long-chain Fluorescein O322 molecules within the monolayer at the
air/water interface was studied by rotating a sample trough using the second-harmonic generation technique.
Azimuthal angle dependences of the polarized second-harmonic intensity (I
pp
, I
ps
, I
ss
, and I
sp
) were measured
for different degrees of monolayer compression. I
pp
and I
ss
showed a 2-fold pattern, whereas I
ps
and I
sp
showed
a 4-fold pattern. A set of theoretical equations was derived to interpret the observed pattern. The observed
patterns agreed with the equations based on C
2
v
symmetry, and the monolayer showed a definite crystalline
structure in the 0.21 nm2/molecule region. These results showed that the transition of an initially disordered
and heterogeneous dye monolayer into a fairly regular crystalline-like structure was characterized by continuous
changes in the structure ordering of constituent molecules maintaining C
2
v
symmetry.
Using a confocal fluorescence microscope, we found a significant temporal fluctuation in the fluorescence spectrum of porphine molecules on the water surface. The spectra fluctuated when the accumulation time was 300 ms and the pH was within a certain range. No fluctuation was observed with a longer accumulation time (36 s) or on a solid surface. The present observation indicates that the distribution of porphine on the water surface is heterogeneous and that the Brownian motion of the surface species induces spectral fluctuation. A neutral form of porphine produces domains at a higher surface coverage.
Mixed insoluble monolayers of bis(N-ethyl,N-octadecyl)rhodamine perchlorate (RhC18) and arachidic
acid (ArAc) at the air−water interface were characterized by surface pressure−area isotherm, second
harmonic generation (SHG), and reflection spectroscopy studies. The analysis of surface pressure−area
isotherms of mixed RhC18/ArAc layers provided evidence that there was a high degree of miscibility of film
components and a strong interaction among them. A new band was found to appear on the blue side of
the reflection spectrum after an increase in both the concentration of ArAc in the layer and the applied
surface pressure. This new band was assigned to dye aggregates, where spontaneous formation in the
gaslike region was promoted by the presence of ArAc and induced by compression in the region of the
continuous monolayer. Both in gaslike and in compressed film, the formation of blue-shifted aggregates
coincided with the increase of the SHG response of the monolayer. Resonance SHG enhancement was
proven to be the main reason for this observation due to electronic resonance of aggregates with the
incident light frequency.
Orientation of pyrenehexadecanoic acid (PyHA) on the water surface has been investigated using surface
compression, fluorescence spectra, and two-photon ionization measurements. PyHA stays as a monomer
at surface densities lower than 0.025 nmol/cm2, while at high surface densities excimer emission was
observed. The two-photon ionization signal had a peak at 90° (p-polarized laser beam) upon the rotation
of the polarization of the incident laser beam. The difference in the signal intensity generated by p-polarized
and s-polarized lights became smaller as surface density increased. It is suggested that the tilt angle of
the transition moment of the pyrene ring is ca. 70° at a low surface density, and it increases with the surface
density increase. Problems in determining orientation of the molecules on water are discussed.
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