In this work, Fourier integral microscope (FIMic), an ultimate design of 3D-integral microscopy, is presented. By placing a multiplexing microlens array at the aperture stop of the microscope objective of the host microscope, FIMic shows extended depth of field and enhanced lateral resolution in comparison with regular integral microscopy. As FIMic directly produces a set of orthographic views of the 3D-micrometer-sized sample, it is suitable for real-time imaging. Following regular integral-imaging reconstruction algorithms, a 2.75-fold enhanced depth of field and [Formula: see text]-time better spatial resolution in comparison with conventional integral microscopy is reported. Our claims are supported by theoretical analysis and experimental images of a resolution test target, cotton fibers, and in-vivo 3D-imaging of biological specimens.
Integral microscopes (IMic) have been recently developed in order to capture the spatial and the angular information of 3D microscopic samples with a single exposure. Computational post-processing of this information permits to carry out a 3D reconstruction of the sample. By applying conventional algorithms, both depth and also view reconstructions are possible. However, the main drawback of IMic is that the resolution of the reconstructed images is low and axially heterogeneous. In this paper, we propose a new configuration of the IMic by placing the lens array not at the image plane, but at the pupil (or Fourier) plane of the microscope objective. With this novel system, the spatial resolution is increased by factor 1.4, and the depth of field is substantially enlarged. Our experiments show the feasibility of the proposed method.
Abstract:Integral-imaging technology has demonstrated its capability for computing depth images from the microimages recorded after a single shot. This capability has been shown in macroscopic imaging and also in microscopy. Despite the possibility of refocusing different planes from one snap-shot is crucial for the study of some biological processes, the main drawback in integral imaging is the substantial reduction of the spatial resolution. In this contribution we report a technique, which permits to increase the two-dimensional spatial resolution of the computed depth images in integral microscopy by a factor of √2. This is made by a doubleshot approach, carried out by means of a rotating glass plate, which shifts the microimages in the sensor plane. We experimentally validate the resolution enhancement as well as we show the benefit of applying the technique to biological specimens.
This paper proposes a method for the generation of high-contrast localized sinusoidal fringes with spatially noncoherent illumination and relatively high light throughput. The method, somehow similar to the classical Lau effect, is based on the use of a Fresnel biprism. It has some advantages over previous methods for the noncoherent production of interference fringes. One is the flexibility of the method, which allows the control of the fringe period by means of a simple axial shift of the biprism. Second is the rapid axial fall-off in visibility around the high-contrast fringe planes. And third is the possibility of creating fringes with increasing or with constant period as the light beam propagates. Experimental verifications of the theoretical statements are also provided.
Integral Imaging provides spatial and angular information of three-dimensional (3D) objects, which can be used both for 3D display and for computational post-processing purposes. In order to recover the depth information from an integral image, several algorithms have been developed. In this paper, we propose a new free depth synthesis and reconstruction method based on the two-dimensional (2D) deconvolution between the integral image and a simplified version of the periodic impulse response function (IRF) of the system. The period of the IRF depends directly on the axial position within the object space. Then, we can retrieve the depth information by performing the deconvolution with computed impulse responses with different periods. In addition, alternative reconstructions can be obtained by deconvolving with non-conventional synthetic impulse responses. Our experiments show the feasibility of the proposed method as well as its potential applications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.