Persistence of the opportunistic bacterial pathogen Vibrio cholerae in aquatic environments is the principal cause for seasonal occurrence of cholera epidemics. This causality has been explained by postulating that V. cholerae forms biofilms in association with animate and inanimate surfaces. Alternatively, it has been proposed that bacterial pathogens are an integral part of the natural microbial food web and thus their survival is constrained by protozoan predation. Here, we report that both explanations are interrelated. Our data show that biofilms are the protective agent enabling V. cholerae to survive protozoan grazing while their planktonic counterparts are eliminated. Grazing on planktonic V. cholerae was found to select for the biofilm-enhancing rugose phase variant, which is adapted to the surface-associated niche by the production of exopolymers. Interestingly, grazing resistance in V. cholerae biofilms was not attained by exopolymer production alone but was accomplished by the secretion of an antiprotozoal factor that inhibits protozoan feeding activity. We identified that the cell density-dependent regulator hapR controls the production of this factor in biofilms. The inhibitory effect of V. cholerae biofilms was found to be widespread among toxigenic and nontoxigenic isolates. Our results provide a mechanistic explanation for the adaptive advantage of surface-associated growth in the environmental persistence of V. cholerae and suggest an important contribution of protozoan predation in the selective enrichment of biofilm-forming strains in the out-of-host environment.grazing ͉ resistance ͉ protozoa ͉ quorum sensing E pidemics of cholera, an acute intestinal infection caused by toxigenic strains of the facultative pathogen Vibrio cholerae, are a major public health problem in developing countries around the globe. Both toxigenic and nontoxigenic strains of V. cholerae are natural inhabitants of a wide range of aquatic ecosystems, including estuarine and coastal waters, that provide the environmental reservoir of virulent V. cholerae strains (1). The fact that many environmental nontoxigenic strains carry virulence genes (2, 3) and that the occurrence of epidemics coincides with the increased prevalence of the causative V. cholerae strain in the aquatic environment (4, 5) supports the notion of an environmental origin of toxigenic V. cholerae clones. This view has led to the hypothesis that cholera epidemics are triggered by environmental factors and selective forces governing aquatic microbial communities. In recent years, studies on the ecology of V. cholerae have considerably increased our understanding of physical and biological parameters that influence the persistence of V. cholerae in the environment and hold the potential to predict the outbreak of cholera epidemics (6).As a member of the natural bacterioplankton community, V. cholerae is an integral part of the pelagic microbial food web and is thus constrained in its growth and survival by the predatory action of bacterivorous protists, so...

The detailed analysis of the International Marine Past Global Changes Study core MD99‐2343 recovered from a sediment drift at 2391 m water depth north of the island of Minorca illustrates the effects of climate variability on thermohaline circulation in the western Mediterranean during the last 12 kyr. Geochemical ratios associated with terrigenous input resulted in the identification of four phases representing different climatic and deepwater overturning conditions in the Western Mediterranean Basin during the Holocene. Superimposed on the general trend, eight centennial‐ to millennial‐scale abrupt events appear consistently in both grain size and geochemical records, which supports the occurrence of episodes of deepwater overturning reinforcement in the Western Mediterranean Basin. The observed periodicity for these abrupt events is in agreement with the previously defined Holocene cooling events of the North Atlantic region, thus supporting a strong Atlantic‐Mediterranean climatic link at high‐frequency time intervals during the last 12 kyr. The rapid response of the Mediterranean thermohaline circulation to climate change in the North Atlantic stresses the importance of atmospheric teleconnections in transferring climate variability from high latitudes to midlatitudes.

Palynological, sedimentological and stable isotopic analyses of carbonates and organic matter performed on the El Portalet sequence (1802 m a.s.l., 42°48′00ʺN, 0°23′52ʺW) reflect the paleoclimatic evolution and vegetation history in the central-western Spanish Pyrenees over the last 30,000 yr, and provide a high-resolution record for the late glacial period. Our results confirm previous observations that deglaciation occurred earlier in the Pyrenees than in northern European and Alpine sites and point to a glacial readvance from 22,500 to 18,000 cal yr BP, coinciding with the global last glacial maximum. The patterns shown by the new, high-resolution pollen data from this continental sequence, chronologically constrained by 13 AMS 14C dates, seem to correlate with the rapid climate changes recorded in Greenland ice cores during the last glacial–interglacial transition. Abrupt events observed in northern latitudes (Heinrich events 3 to 1, Oldest and Older Dryas stades, Intra-Allerød Cold Period, and 8200 cal yr BP event) were also identified for the first time in a lacustrine sequence from the central-western Pyrenees as cold and arid periods. The coherent response of the vegetation and the lake system to abrupt climate changes implies an efficient translation of climate variability from the North Atlantic to mid latitudes.

A multiproxy study of sediments cores from Zoñar Lake (37º29'00''N, 4º41'22'' W, 300 m a.s.l.) supported by 11 14 C AMS dates provides the first high-resolution centennial-scale reconstruction of past humidity changes in southern Spain during the last 4000 years. Arid periods occurred prior to 2.9 cal. kyr BP and during 1.3-0.6 cal. kyr BP (Medieval Climate Anomaly). The most humid period occurred during 2.6-1.6 cal. kyr BP encompassing the late Iron Age-Iberian and Roman epochs. Two humid periods of lower intensity occurred between 0.8-0.6 cal kyr BP (1200-1400 AD) and about 400 cal. yr BP (around 1600 AD) coinciding with the onset of the Little Ice Age. Humid conditions are synchronous with a decline in solar output and seem to correspond to atmospheric patterns similar to negative NAO phases. Arid conditions show better correlation with northern Africa climate evolution suggesting a possible link to subtropical dynamics. The geographic location of Zoñar Lake and the robust chronology provides an opportunity to improve our understanding of the climate evolution in mid latitudes during the Late Holocene and to evaluate subtropical and high latitude factors in Mediterranean climate evolution.

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Millennial to submillennial marine oscillations that are linked with the North Atlantic's Heinrich events and Dansgaard–Oeschger cycles have been reported recently from the Alboran Sea, revealing a close ocean-atmosphere coupling in the Mediterranean region. We present a high-resolution record of lithogenic fraction variability along IMAGES Core MD 95-2043 from the Alboran Sea that we use to infer fluctuations of fluvial and eolian inputs to the core site during periods of rapid climate change, between 28,000 and 48,000 cal yr B.P. Comparison with geochemical and pollen records from the same core enables end-member compositions to be determined and to document fluctuations of fluvial and eolian inputs on millennial and faster timescales. Our data document increases in northward Saharan dust transports during periods of strengthened atmospheric circulation in high northern latitudes. From this we derive two atmospheric scenarios which are linked with the intensity of meridional atmospheric pressure gradients in the North Atlantic region.

During the period of instrumental records, the North Atlantic Oscillation (NAO) has strongly influenced interannual precipitation variations in the western Mediterranean, while some eastern parts of the basin have shown an anti-phase relationship in precipitation and atmospheric pressure. Here we explore how the NAO and other atmospheric circulation modes operated over the longer timescales of the Medieval Climate Anomaly (MCA) and Little Ice Age (LIA). High-resolution palaeolimnological evidence from opposite ends of the Mediterranean basin, supplemented by other palaeoclimate data, is used to track shifts in regional hydro-climatic conditions. Multiple geochemical, sedimentological, isotopic and palaeoecological proxies from Estanya and Montcortés lakes in northeast Spain and Nar lake in central Turkey have been crosscorrelated at decadal time intervals since AD 900. These dryland lakes capture sensitively changes in precipitation/evaporation (P/E) balance by adjustments in water level and salinity, and are especially valuable for reconstructing variability over decadal-centennial timescales. Iberian lakes show lower water levels and higher salinities during the 11th to 13th centuries synchronous with the MCA and generally more humid conditions during the 'LIA' (15th-19th centuries). This pattern is also clearly evident in tree-ring records from Morocco and from marine cores in the western Mediterranean Sea. In the eastern Mediterranean, palaeoclimatic records from Turkey, Greece and the Levant show generally drier hydro-climatic conditions during the LIA and a wetter phase during the MCA. This implies that a bipolar climate see-saw has operated in the Mediterranean for the last 1100 years. However, while western Mediterranean aridity appears consistent with persistent positive NAO state during the MCA, the pattern is less clear in the eastern Mediterranean. Here the strongest evidence for higher winter season precipitation during the MCA comes from central Turkey in the northeastern sector of the Mediterranean basin. This in turn implies that the LIA/MCA hydroclimatic pattern in the Mediterranean was determined by a combination of different climate modes along with major physical geographical controls, and not by NAO forcing alone, or that the character of the NAO and its teleconnections have been non-stationary.

The multi-proxy analysis of sediment cores recovered in karstic Lake Estanya (42°02' N, 0°32' E; 670 m a. s. l., NE Spain), located in the transitional area between the humid Pyrenees and the semi-arid Central Ebro Basin, provides the first high-resolution, continuous sedimentary record in the region, extending back the last 21 000 years. The integration of sedimentary facies, elemental and isotopical geochemistry and biogenic silica, together with a robust age model based on 17 AMS radiocarbon dates, enables precise reconstruction of the main hydrological and environmental changes in the region during the last deglaciation. Arid conditions, represented by shallow lake levels, predominantly saline waters and reduced organic productivity occurred throughout the Last Glacial Maximum (21-18 cal kyrs BP) and the late glacial, reaching their maximum intensity during the period 18-14.5 cal kyrs BP (including Heinrich event 1) and the Younger Dryas (12.9-11.6 cal kyrs BP). Less saline conditions characterized the 14.5-12.6 cal kyrs BP period, suggesting higher effective moisture during the Bölling/Allerød. The onset of more humid conditions started at 9.4 cal kyrs, indicating a delayed hydrological response to the onset of the Holocene which is also documented in several sites of the Mediterranean Basin. Higher, although fluctuating, Holocene lake levels were punctuated by a mid Holocene arid period between 4.8 and 4.0 cal kyrs BP. A major lake-level rise occurred at 1.2 cal kyrs BP, conducive to the establishment of conditions similar to the present and interrupted by a last major water level drop, occurring around 800 cal yrs BP, which coincides with the Medieval Climate Anomaly. The main hydrological stages in Lake Estanya are in phase with most Western Mediterranean and North Atlantic continental and marine records, but our results also show similarities with other Iberian and northern African reconstructions, emphasizing peculiarities of palaeohydrological evolution of the Iberian Peninsula during the last deglaciation.