Herein we present a geographical distribution map of Gastrotheca albolineata, an endemic species of marsupial treefrogs from ombrophilous formations in the Atlantic Forest biome. The new records were provided from field observations, collected specimens and data from museum specimens. The present study report new records from Rio de Janeiro state, provide an updated distribution map, and comments on its ecological aspects, thus contributing to future studies concerning the biogeography and biology of this group. Considering the lack of studies on the biology of this species as well as the lack of knowledge on its population trends, we also suggest changes in the status of conservation of the species by the IUCN.
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