Chronic kidney disease (CKD) patients develop anemia because of the low kidney erythropoietin (EPO) production, thus promoting cardiovascular complications. The degree of renal insufficiency might determine the moment to start recombinant human erythropoietin (rhEPO) therapy, but the molecular basis for these options deserves better elucidation. This study aimed to clarify the cardio-renal effects of earlier rhEPO therapy in rats with moderate chronic renal failure (CRF). Four groups of rats were evaluated for 15 weeks (control; rhEPO − 50 IU/kg/week; CRF − 3/4 nephrectomy; CRF + rhEPO) to assess renal and hematology data, EPO levels, blood pressure, heart rate, peripheral catecholamines contents, serum-transforming growth factor-b1 (TGF-b1), kidney gene expression of EPO, Caspase 9 (Casp9), and vascular endothelial growth factor (Vegf). This model of moderate CRF showed moderate and corrected anemia, hypertension, tachycardia, sympathetic overactivity, and increased serum TGF-b1 content. The remnant kidney showed a proliferative profile, with hypertrophy, downregulated gene expression of EPO, and upregulated gene expression of Vegf and Casp9. rhEPO treatment promoted erythrocytosis and prevented tachycardia and catecholamines increment, with a rise of serum TGF-b1. Furthermore, the decreased kidney gene expression of EPO and the overexpression of Casp9 were prevented, demonstrating a renoprotective action on the remnant kidney. In conclusion, rhEPO therapy promotes a protective effect on the cardio-renal axis, which might be mainly attributed to its pro-proliferative and anti-apoptotic properties. These findings might recommend its use in earlier stages of CRF, acting as an erythropoiesis stimulating agent, to efficiently correct not only the anemia, one of the major complications in these patients, but also the succeeding adverse cardio-renal effects.

The concern for environmental protection within an increasingly globalised international system has led to the planning of Transfrontier Conservation Areas, among which Peace Parks aim to achieve or maintain peace across borders. Based on the growing importance that tourism practice has for the sustainability of Peace Parks, this chapter reflects on how the tourism activity developed within Peace Parks can contribute to peacebuilding processes. This dynamic is addressed by analysing a case study, focused on the Great Limpopo Transfrontier Park. It shows that tourism has the potential to positively contribute to sustainability, creating better living conditions for the local population and, consequently, promoting the achievement of peace. However, it also shows that tourism may have the opposite effect if national interests dominate, if there is insufficient consultation of local communities' interests or if sensitive border and land-use issues are not overcome.

The concern for environmental protection within an increasingly globalised international system has led to the planning of Transfrontier Conservation Areas, among which Peace Parks aim to achieve or maintain peace across borders. Based on the growing importance that tourism practice has for the sustainability of Peace Parks, this chapter reflects on how the tourism activity developed within Peace Parks can contribute to peacebuilding processes. This dynamic is addressed by analysing a case study, focused on the Great Limpopo Transfrontier Park. It shows that tourism has the potential to positively contribute to sustainability, creating better living conditions for the local population and, consequently, promoting the achievement of peace. However, it also shows that tourism may have the opposite effect if national interests dominate, if there is insufficient consultation of local communities' interests or if sensitive border and land-use issues are not overcome.