Os rizomas de Mariscus pedunculatus, quando submetidos à hidrodestilação, forneceram uma mistura do diidrobenzofurano remirol e do benzopirano iso-evodionol, na proporção de 5.7:1. Estas substâncias foram separadas e purificadas por intermédio de sucessivas cromatografias de camada delgada preparativa. As estruturas foram confirmadas por métodos espectroscópicos, incluindo técnicas bidimensionais de RMN, e pelo produto de hidrogenação, no caso do remirol. Entre as substâncias voláteis, foram identificados traços de outros oito sesquiterpenos por CG-EM associada aos cálculos de índice de retenção. O hidrodestilado bruto, assim como as substâncias purificadas foram testadas quanto à atividade antiinflamatória, no modelo de pleurisia induzida em camundongos por lipopolissacarídeo (LPS, 12,5 ng/cavidade). Tanto o extrato bruto quanto o remirol puro (100 e 200 mg kg-1 , via oral) demonstraram uma capacidade dose-dependente de inibir o acúmulo de neutrófilos e eosinófilos na cavidade pleural dos animais. O iso-evodionol não apresentou efeito antiinflamatório. A mixture of the benzofuran remirol and the benzopyran isoevodionol (5.7:1) was extracted by hydrodistillation from rhizomes of Mariscus pedunculatus. They were purified by successive thin layer chromatography. Their structure elucidation were confirmed by 2D-NMR experiments. The structure of remirol was also confirmed by its hydrogenation product. Crude distillate and the pure compounds were screened for anti-inflammatory activity by the use of the mouse pleurisy model induced by lipopolysaccharide (LPS, 12.5 ng/cavity). Both the mixture and remirol showed a dosedependent (100 and 200 mg kg-1 p. o.) capacity to inhibit neutrophil and eosinophil accumulation in the mouse pleural cavity. In contrast, no anti-inflammatory effect was observed for iso-evodionol.
Through active surveillance and contact tracing from outpatients, we aimed to identify and characterize SARS‐CoV‐2 variants circulating in Porto Velho‐Rondônia, a city in the Brazilian Amazon. As part of a prospective cohort, we gathered information from 2,506 individuals among COVID‐19 patients and household contacts. Epidemiological data, nasopharyngeal swabs, and blood samples were collected from all participants. Nasopharyngeal swabs were tested for antigen rapid diagnostic test and reverse transcription‐polymerase chain reaction (RT‐PCR) followed by genomic sequencing. Blood samples underwent ELISA testing for IgA, IgG, and IgM antibody levels. From 757 specimens sequenced, three were identified as Mu variant, none of the individuals carrying this variant had a travel history in the previous 15 days before diagnosis. One case was asymptomatic and two presented mild symptoms. Two infected individuals from different households caring viruses with additional amino acid substitutions ORF7a P45L and ORF1a T1055A compared to the Mu virus reference sequence. One patient presented IgG levels. Our results highlight that genomic surveillance for SARS‐CoV‐2 variants can assist in detecting the emergency of SARS‐CoV‐2 variants in the community, before its identification in other parts of the country.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.