Asparaginase catalyzes the degradation of L-asparagine to L-aspartic acid and ammonia, and is implicated in the catabolism of transported asparagine in sink tissues of higher plants. The Arabidopsis genome includes two genes, ASPGA1 and ASPGB1, belonging to distinct asparaginase subfamilies. Conditions of severe nitrogen limitation resulted in a slight decrease in seed size in wild-type Arabidopsis. However, this response was not observed in a homozygous T-DNA insertion mutant where ASPG genes had been inactivated. Under nitrogen-sufficient conditions, the ASPG mutant had elevated levels of free asparagine in mature seed. This phenotype was observed exclusively under conditions of low illumination, when a low ratio of carbon to nitrogen was translocated to the seed. Mutants deficient in one or both asparaginases were more sensitive than wild-type to inhibition of primary root elongation and root hair emergence by L-asparagine as a single nitrogen source. This enhanced inhibition was associated with increased accumulation of asparagine in the root of the double aspga1-1/-b1-1 mutant. This indicates that inhibition of root growth is likely elicited by asparagine itself or an asparagine-derived metabolite, other than the products of asparaginase, aspartic acid or ammonia. During germination, a fusion between the ASPGA1 promoter and beta-glucuronidase was expressed in endosperm cells starting at the micropylar end. Expression was initially high throughout the root and hypocotyl, but became restricted to the root tip after three days, which may indicate a transition to nitrogen-heterotrophic growth.

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Аннотация. В статье рассматриваются семантические аспекты хронотопа современной свадьбы в сравне-нии с традиционной свадебной обрядностью. Применение структурно-семиотического подхода позволило выделить следующие трансформации: упрощение темпорального символизма при сохранении традицион-ной трехчленной структуры; утрату биполярности пространственной структуры; повышение значимости публичного пространства, ориентацию на информационный отклик.Summary. The article considers the semantic aspects of modern wedding chronotopos compared to the traditional wedding rites. The use of structural and semiotic approach allows finding out the following transformations: simplification of temporal symbolism with maintaining the traditional tripartite structure; loss of spatial structure bipolarity; increasing importance of public space; focus on informative cue.Ключевые слова: хронотоп, семантика, свадебная обрядность, инициация, обряды перехода.

The aim of this research is to determine whether there is any statistically signifi cant diff erence in motor abilities of boys and girls of younger school age. The sample includes 76 examinees with 37 males and 39 females. All are 5th and 6 th graders of primary school. The evaluation of motor abilitied is based on 5 tests. Multivariant analysis of variance is applied for determination of diff erences within the whole system of motor tests, whereas univariant analysis of variance is used for determining diff erences in each motor test. According to the fi ndings, statistically signifi cant diff erences in motor abilities between boys and girls at the studied age can be noticed in the test 20 m running from fl ying start, which is in favour of boys and in the test sit and reach, in favour of girls, while the diff erences in other tests are not statistically signifi cant. Considering the evaluation of motor abilities, boys have statistically much better results in the speed of alternative movements and explosive strenth, whereas girls have achieved much better results in the test for fl exibility. It has also been concluded that 5th and 6th graders have statistically better results in the space of coordination and explosive strenght, whereas girls are much better in the fi eld of fl exibility and suppleness.

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