Stanojević et al., Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative ...
We investigated the effect of disulfiram (DSF) on reproductive toxicity induced by subchronic exposure to cadmium (Cd). We examined the redox status and systemic testosterone changes in the testes and plasma of Cd-treated male Wistar rats. Rats were treated with 1 mg CdCl 2 /kg body weight (bw)/day (intraperitoneal administration) for 42 days; in the second experimental group, rats were given 178.5 mg DSF/kg bw/day by oral gavage for 21 days; in the third group, after administration of Cd for 21 days, DSF treatment was introduced on day 22 and lasted until day 42, with continuous Cd intake. Each experimental group had a matching control: untreated rats, rats that received for 21 days olive oil, the solvent for DSF; rats that started with olive oil intake from days 22-42. Exposure of rats to DSF modulated the oxidative status in the testes; thus, coexposure increased the Cd-induced reduction in total superoxide dismutase (tSOD), catalase (CAT), glutathione reductase (GR) and total glutathione-S-transferase (tGST) activities, and lowered the Cd-increased superoxide anion radical (O 2 •-) and malondialdehyde (MDA) concentrations. DSF did not affect testosterone production diminished by Cd, as Leydig cells, once impaired by Cd, could not be reactivated by DSF.
Background / Aim. The goal of the study was to test if subacute administration of micronized zeolite (MZC) accomplishes radioprotective role based on the evaluation of the status of oxidative stress (OS) in the brain and 8-hydroxyguanosine (8-OH-dG) in plasma of rats exposed to the single γ-ray irradiation of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a four-week normal or 5% MZC supplemented diet and afterward exposed to the single γ-ray irradiation of 2 and 10Gy. Groups of rats: a) on a normal diet: the control group and 2Gy and 10Gy groups; b) on 5% MZC supplemented diet: the control group-MZC, MZC+2Gy, and MZC+10Gy groups. We measured malondialdehyde (MDA), glutathione (GSH) total, and activity of total and manganese superoxide dismutase (tSOD and MnSOD) in vulnerable brain regions (cerebellum, hippocampus, and cerebral cortex) and 8-OH-dG in plasma. Results. Lower MDA was lower in MZC+2Gy and MZC+10Gy compared to 2Gy and 10Gy groups. Total SOD was higher in MZC+10Gy than in 10Gy. GSH was the highest in the 10Gy group. Compering to the control group, 8-OH-dG was extremely higher in groups radiated with 10 Gy regardless of diet, but slightly lower in MZC+2Gy and 2Gy groups. Conclusion. Subacute MZC pretreatment accomplished partial radioprotective effect in rats based on suppressed SOD activity compared to non-irradiated rats at 2 Gy, and reduced brain MDA at 2 and 10 Gy.
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