PurposeThe majority of ISO 9001 certification research studies conducted so far are supported by survey methodologies and descriptive statistics. As such, they express conclusions that are mainly derived from opinions and perceptions about the subject. Thus, it is common to find in the open literature references that point out the highly subjective results derived from such studies (often of somewhat contradictory nature). This paper tries to compile the main conclusions that ISO 9001 research studies have tried to address, with the aim of describing the quality management systems literature state‐of‐the‐art.Design/methodology/approachIn order to achieve this objective, an exhaustive literature review of ISO 9000 studies was carried out. For that purpose approximately 100 articles were analysed, which were sorted in the following categories, according to the used methodology: surveys; analysis of financial indicators; case studies; interviews; literature review; and statistical data analysis.FindingsThis paper reflects an exhaustive literature review, which allowed the identification of a group of issues that ISO 9001 research studies have tried to address, as well as the most commonly used methodologies employed and main conclusions reached so far by the different authors. Then using such a state‐of‐the‐art as a starting point, it pointed out a number of questions that seem to be relevant but have not been so far addressed in the open literature in terms of providing clear and precise answers, as well as others that are at the core of the authors' future work in this area.Originality/valueThe value of the paper is to point out and to compile the main conclusions reached by the different authors and researchers in the most important ISO 9001 issues addressed in the open literature.

Antimicrobial peptides (AMPs) are part of the innate immune system and are generally defined as cationic, amphipathic peptides, with less than 50 amino acids, including multiple arginine and lysine residues. The human cathelicidin antimicrobial peptide LL37 can be found at different concentrations in many different cells, tissues and body fluids and has a broad spectrum of antimicrobial and immunomodulatory activities. The healing of wound is a complex process that involves different steps: hemostasis, inflammation, remodeling/granulation tissue formation and re-epithelialization. Inflammation and angiogenesis are two fundamental physiological conditions implicated in this process. We have recently developed a new method for the expression and purification of recombinant LL37. In this work, we show that the recombinant peptide P-LL37 with a N-terminus proline preserves its immunophysiological properties in vitro and in vivo. P-LL37 neutralized the activation of macrophages by lipopolysaccharide (LPS). Besides, the peptide induced proliferation, migration and tubule-like structures formation by endothelial cells. Wound healing experiments were performed in dexamethasone-treated mice to study the effect of LL37 on angiogenesis and wound regeneration. The topical application of synthetic and recombinant LL37 increased vascularization and re-epithelialization. Taken together, these results clearly demonstrate that LL37 may have a key role in wound regeneration through vascularization.

PurposeTo determine the current state of knowledge related to performance measures and their degree of implementation in small and medium enterprises (SMEs) in England.Design/methodology/approachThe paper starts with a literature review and then goes on to discuss the methodology used. The survey is briefly presented together with the analysis of the resultant data. General opinions regarding performance measurement in English SMEs are described, including the most important measures and the biggest obstacles to the adoption of new ones. Hypotheses about differences between groups are tested and discussed.FindingsThis work concludes that there is a gap between the theory/knowledge of performance measures and the practice in English SMEs. Training of employees and difficulty in defining new performance measures were highlighted as the major obstacles to the adoption of new performance measures.Research limitations/implicationsThe low response rate of the survey precludes the generalisation of the findings.Practical implicationsInnovation and learning measures should be applied more widely.Originality/valueThis paper is relevant to academics and SME managers because it supports the existence of a gap between the theory of performance measurement and its degree of implementation. In addition, it introduces both theoretical information on performance measurement, including that based on the balanced scorecard perspectives, and practical information from a survey conducted in English SMEs.

The hydrolysis/fermentation of wheat straw and the adsorption/desorption/deactivation of cellulases were studied using Cellic(®) CTec2 (Cellic) and Celluclast mixed with Novozyme 188. The distribution of enzymes - cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase - of the two formulations between the residual substrate and supernatant during the course of enzymatic hydrolysis and fermentation was investigated. The potential of recyclability using alkaline wash was also studied. The efficiency of hydrolysis with an enzyme load of 10 FPU/g cellulose reached >98% using Cellic(®) CTec2, while for Celluclast a conversion of 52% and 81%, was observed without and with β-glucosidase supplementation, respectively. The decrease of Cellic(®) CTec2 activity observed along the process was related to deactivation of Cel7A rather than of Cel7B and β-glucosidase. The adsorption/desorption profiles during hydrolysis/fermentation revealed that a large fraction of active enzymes remained adsorbed to the solid residue throughout the process. Surprisingly, this was the case of Cel7A and β-glucosidase from Cellic, which remained adsorbed to the solid fraction along the entire process. Alkaline washing was used to recover the enzymes from the solid residue. This method allowed efficient recovery of Celluclast enzymes; however, this may be achieved only when minor amounts of cellulose remain present. Regarding the Cellic formulation, neither the presence of cellulose nor lignin restricted an efficient desorption of the enzymes at alkaline pH. This work shows that the recycling strategy must be customized for each particular formulation, since the enzymes found e.g. in Cellic and Celluclast bear quite different behaviour regarding the solid-liquid distribution, stability and cellulose and lignin affinity.

The recovery of cellulases from lignin, lignocellulosic hydrolysates and cellulose by alkaline washes at pH 9 and 10 was examined. The effect of the pH on the structural stability of purified Cel7A was analyzed by circular dichroism. Purified Cel7A showed conformational changes at pH 9 and 10 that were reversible at pH 4.8. Temperature influenced the enzymatic hydrolysis of wheat straw and may be critical for the efficiency of cellulase recycling from wheat straw hydrolysates. Operation at moderate temperatures (37°C) resulted in a rate of saccharification 19% higher than that obtained at 50° C, improving cellulase recycling by 49%. Over 60% of the enzyme activity on the synthetic substrate 4-methylumbelliferyl-β-d-cellobioside (MUC) may be recovered by using a simple alkaline wash. This is thus a promising strategy for enzyme recycling that is simple to implement at industrial scale, economical and effective.

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This paper describes a survey that was undertaken at the initial stage of a research project looking at performance measures and quality tools. Its objective was to determine the level of knowledge of performance measures and their degree of implementation in small and medium enterprises (SMEs) in Portugal. It also addressed the level of use of quality tools in the same companies, since they provide some degree of integration between the external requirements of a business and its internal activities and performance (Gregory, 1993). Most research to date has concentrated on large companies and so an important part of this work is to assess whether or not SMEs can adopt similar approaches with respect to quality, or in general, to management methodologies. For example, are ISO standards, TQM, balanced scorecard, or other excellence models beneficial to all companies and how are they implemented? The paper starts with a brief literature review and then presents the methodology used for the survey, the questionnaire construction and the pilot study. The full survey is described together with a comprehensive analysis of the results, including respondent bias and questionnaire reliability and validity. The general opinions of Portuguese SMEs with regard to performance measurement, the most important measures to use and the biggest obstacles to their adopting new ones are discussed. The paper culminates with an analysis of quality tools and the conclusions drawn from the study.

Bacterial cellulose (BC) is a biopolymer synthesized by certain acetic acid bacteria strains. The safety of BC regarding its potential use in food applications is here reviewed. The acute, sub-acute and subchronic oral toxicity assays showed that consumption of BC had no adverse effects in rats. Several studies demonstrated that BC is not genotoxic, did not induce chromosomal aberrations in CHO cells under both non-activating and metabolic activating conditions, is inactive in the in vitro Rat Primary Hepatocyte Unscheduled DNA Synthesis Assay, had no reproductive toxicity in mice and exerted no embryotoxicity and teratogenicity effects in rats.Several studies on the BC in biomedical applications further reinforces its safety: a primary eye and dermal irritation studies in the rabbit showed that BC was non-irritating. The inflammatory reaction to subcutaneously implanted BC has been evaluated in animal models and for different periods of time, demonstrating that BC is biocompatible and does not trigger a harsh inflammatory reaction.Altogether, and considering its longstanding history of human consumption in Asian countries, as well as its utilization in biomedical devices, it may be concluded that BC is safe for applications in food technology.