ABSTRACT.-Corrêa E.S., Paludo G.R., Scalon M.C., Machado J.A., Lima A.C.Q., Pinto A. Ehrlichia sp. and Anaplasma platys are Gram-negative micro-organisms, obligate intracellular parasites, residing in cytoplasmic vacuoles of leukocytes and platelets, found in peripheral blood or tissue. Few reports have been made about ehrlichiosis and anaplasmosis in cats in Brazil, which are based on the presence of morulae in leukocytes and platelets, or by detecting antibodies. The objective of this study was to investigate the natural infection with Ehrlichia sp. and A. platys in cats in Campos dos Goytacazes-RJ, by hematoscopia and DNA detection of these agents. Samples of whole blood and serum from 91 cats, regardless of race, gender and age. Blood count, serum biochemistry and PCR using primers for Ehrlichia sp. and A. platys were perfomed. Data from hematoscopia showed 9.89% of morulae only in platelets. The DNA of A. platys was detected in 13.18% of the 91 samples and 44.44% of the positive at hematoscopia. The DNA of Ehrlichia sp. was not detected in any sample. All animals studied did not show clinical signs neither positive laboratory results. The data suggest that domestic cats can serve as potential reservoirs for A. platys as asymptomatic form of related diseases.
ResumoEste trabalho tem como finalidade divulgar a utilização de Dermestes maculatus na preparação de material osteológico de diferentes espécies animais e, ao mesmo tempo, contribuir para a diversificação da coleção osteológica da Seção de Anatomia da UENF. Seis animais de diferentes espécies tiveram seus esqueletos limpos pelos insetos. O procedimento de limpeza completa foi obtido em diferentes períodos de tempo e mantinha uma relação direta com o número de larvas e insetos adultos presentes nas colônias. No final da exposição, alguns
entre eles o divertículo vesico-uracal. Este artigo relata o caso de uma cadela, adulta, que apresentava episódios recorrentes de cistite e durante algum tempo de tratamento apresentou boa resposta à antibioticoterapia. Durante um exame ultrassonográfico foi possível observar a presença de uma vesícula urinária com parede espessa e irregular, com imagem sugestiva de cistite. Junto a esta, foi visualizada uma grande formação cística sugestivo de divertículo vesico-uracal.Durante a laparotomia exploratória o provável diagnóstico foi confirmado e procedeu-se a extirpação do segmento uracal.Palavras-Chave: Cão; vesícula urinária; divertículo vesico-uracal Chronic cystitis related to vesico-urachal diverticulum in dog
Acute kidney injury (AKI) is defined as an increase greater than 0.3 mg/L of serum creatinine within 48 hours and is a major cause of death in patients in intensive care units. Twenty-four Wistar rats were divided into three groups: Control (0.9% saline), Genta (gentamicin 50 mg.kg-1 BID) and Deh+Genta (gentamicin 50 mg.kg-1 BID + water restriction) and tested in an AKI model by aminoglycoside administration and dehydration implementation. The animals in the Deh+Genta group exhibited the lowest average weight and feed intake after the fifth day of the experiment. In this same period, water consumption by the Genta group was lower than the Control group, but in the following days of the experiment, polydipsia was noted for this group. The Deh+Genta group displayed the highest mean serum urea after the fifth day. The gentamicin-treated groups exhibited higher means than the Control group for serum creatinine, which proved to be a late renal marker for AKI. Serum GGT was higher in the Deh+Genta group, whereas urinary GGT was higher in the groups that received gentamicin, characterizing enzymuria, although severe dehydration can mask the results by indicating false negative values. The urinary GGT enzyme did not act as an early AKI biomarker. Decreased glomerular filtration rates enhanced the concentration of blood components and masked urinary and tissue components.
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