In December 2019, a new disease caused by a coronavirus was first identified in China and quickly spread across the world, causing a large number of deaths. Quarantine has been implemented in many countries as a measure to prevent the spread of the disease. The impact of this situation on drug use and mental health is still poorly understood. Therefore, this cross-sectional study sought to assess possible associations between quarantine, the use of psychoactive substances, and symptoms of depression and anxiety. The assessment instruments used were the Patient Health Questionnaire-9 (PHQ-9); the General Anxiety Disorder-7 (GAD-7) scale; and an adaptation of the Alcohol, Smoking and Substance Involvement Screening Test (ASSIST). The sample consisted of 2398 participants who met the following inclusion criteria: being in quarantine, aged 18 years or older, and living in Brazil. There was a decrease in the frequency of use of practically all drugs investigated in the evaluated sample. However, some factors were associated with less variations in the frequency of substance use before and during quarantine. These factors were being male, single, and having an occupation different from homemaker.
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