Wound healing is characterized with a dynamic physiological process that must maintain the integrity of the lesion. This study aimed to evaluate morphometrically through in vivo testing, the healing of skin wounds with topical use of Prosopis Juliflora extracts. 27 male and adults Wistar rats, divided into three groups in which they were applied, topical and separately physiological solution of NaCl 0.9 % (GI) , aqueous extract of leaves of P. Juliflora (GII) and aqueous extract of stem bark of P. Juliflora (GIII) in periods of 7, 14 and 21 days. Morphometric evaluation of the wound was done daily and its evolution was followed by observing the appearance of the lesion and extent of scar retrac-tion with a digital caliper. The aqueous extracts of the leaves and stem bark of Prosopis juliflora allow a gradual and stable behavior healing of the wound, and showed satisfactory re-epithelialization process from the viewpoint of granulation tissue starting from the 7th day. We emphasize the importance of expanding experimental animal studies with these compounds, since the data obtained are incipient for their applicability in human beings.
RESUMOO câncer é a segunda principal causa de morte no mundo e entre os muitos avanços recentes na quimioterapia contra a doença, os produtos naturais oriundos de plantas têm desempenhado um papel importante. O objetivo deste estudo foi investigar a atividade antitumoral de Prosopis juliflora contra células tumorais humanas MDA-MB-231 (tumor de mama) e SKOV-3 (tumor de ovário) para buscar novos medicamentos derivados de fontes naturais. Testes in vitro foram conduzidos com os extratos brutos de folhas e de casca de caule frente as linhagens de células tumorais MDA-MB-231 e SKOV-3 através do teste de citotoxicidade utilizando MTT (3-(4,5-dimetiazol-2il) 2,5-difenil tetrazólio de brometo) através da monitorização após 24, 48 e 72 h de exposição das células tumorais e não tumorais a diferentes concentrações dos extratos (100; 50; 25; 12,5 μg / mL). Os testes de citotoxicidade também foram realizados contra células não tumorais (fibroblastos). O extrato de casca de caule de P. juliflora inibiu a proliferação de células MDA-MB-231 (28,9%) e não inibiu a proliferação de linhas de células SKOV-3. Este extrato apresentou baixa toxicidade frente as células de fibroblasto. Em contraste, o extrato de folhas de P. juliflora não foi ativo em nenhuma linhagem celular testada. O estudo químico para extração e isolamento de compostos ativos oriundo do extrato da casca de caule é recomendado para ensaios in vitro desses compostos para investigar a atividade antitumoral. Palavras-chaves: Atividade antitumoral; Produtos naturais; Prosopis juliflora. ABSTRACTCancer is the second leading cause of death in the world, among many recent advances in cancer chemotherapy, plant natural products have played an important role. The aim of this study was investigated the antitumor activity of Prosopis juliflora against human tumor cells lines MDA-MB-231 (breast tumor) and SKOV-3 (ovarian tumor) to search for new drugs derived from natural sources. In vitro test was conducted with crude extract foliar and stem bark against tumor cells lines MDA-MB-231and SKOV-3 through in using MTT (3-(4,5-dimethythiazol-2yl)2,5-diphenyl tetrazolium bromide) based cytotoxicity monitoring after 24, 48 and 72 h exposure of the MOLT-4 cells to different concentration of the extract ranging from 100; 50; 25; 12.5 µg/mL. The cytotoxicity assays too were realized against cells fibroblast. The extract of stem bark of P. juliflora inhibited the proliferation of cells lines MDA-MB-231(28.9%) and don't inhibited the proliferation of cells lines SKOV-3. This extract presented low toxicity in cells human fibroblast. By contrast the extract of leaves of P. juliflora were not active in any tested cells lineage. Chemical study to extraction and isolation of actives compounds of extract of stem bark is recommended to in vitro assays these compounds to investigate the antitumor activity.
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