PURPOSE:To extract the Melaleuca sp. oil and to assess its in vitro inhibitory effect against Staphylococcus aureus isolates obtained from lower limb wounds and resistant to several antibiotics. METHODS:A total of 14 test-tubes containing Mueller-Hinton broth were used to determine the Minimum Inhibitory Concentration (MIC). The following concentrations of the Melaleuca sp. oil were added to the first 11 tubes: 8; 4; 2; 1; 0.5; 0.2; 0.1; 0.05; 0.025; 0,0125 and 0.00625%. The 12 th and 13 th tubes, with and without oil, were used as the positive and negative controls, respectively. The experimental study was carried out in triplicate at 37ºC for 18 hours. The Minimum Bactericidal Concentration (MBC), able of killing all the microorganisms, was also determined. Two S. aureus isolates were obtained from lower limb wounds of female patients and the identification of the microorganisms (Staphylococcus aureus) and the test for susceptibility to the antimicrobial agents were carried out by automation using the apparatus MicroScan ® . After identification, the isolates were preserved in liquid Trypticase Soy medium, and inoculated for determination of the MIC and MBC. RESULTS:The MIC was 0.2% and the MBC was 0.4%. CONCLUSION:The Melaleuca sp. oil showed antimicrobial properties in vitro against strains isolated from lower limb wounds which were resistant to multiple antibiotics.
Fixation of the short-term central venous catheter. A comparison of two techniques 1Abstract Purpose: To compare the fixation of the central venous catheter (CVC) using two suture techniques. Methods: A clinical, analytical, interventional, longitudinal, prospective, controlled, singleblind and randomized study in adult, intensive care unit (ICU) patients. After admission and indication of CVC use, the patients were allocated to the Wing group (n = 35, catheter fixation with clamping wings and retainers) or Shoelace group (n = 35, catheter fixation using shoelace cross-tied sutures around the device). Displacement, kinking, fixation failure, hyperemia at the insertion site, purulent secretion, loss of the device, psychomotor agitation, mental confusion, and bacterial growth at the insertion site were evaluated. Results: Compared with the Wing group, the Shoelace group had a lower occurrence of catheter displacement (n=0 versus n =4; p = 0.04), kinking (n=0 versus n=8; p=0.001), and fixation failure (n=2 versus n=8; p=0.018). No significant difference was found in bacterial growth (n=20 versus n=14; p=0.267) between groups. Conclusion:The Shoelace fixation technique presented fewer adverse events than the Wing fixation technique.
Purpose To assess the effect of antibiotic prophylaxis on surgical site infection (SSI) rates in women undergoing breast cancer surgery in two tertiary hospitals in Brazil. Methods This was a randomized, double-blind, placebo-controlled, parallel-group clinical trial. A total of 124 women without independent risk factors for SSI were randomly assigned to receive either cefazolin (antibiotic group, n = 62) or placebo (control group, n = 62) as preoperative prophylaxis. After surgery, all surgical wounds were examined once a week, for four weeks, according to the Centers for Disease Control and Prevention definitions and classifications for SSI. Results Baseline characteristics were homogeneous between the two groups. Only one patient in the antibiotic group developed SSI, which was classified as superficial incisional. The overall SSI rate was low, with no significant difference between groups. Conclusion Antibiotic prophylaxis had no significant effect on reducing SSI rates in women without independent risk factors for SSI undergoing breast cancer surgery.
Modelo do Estudo: Foi realizado um estudo individual, analítico, observacional, longitudinal prospectivo, controlado, estudo de coortes concorrente, realizado no período de abril de 2013 a novembro de 2014. Objetivo: Avaliar a microbiota presente no exudato da úlcera venosa de pacientes com “Bota de Unna” e a sua resistência aos antimicrobianos. Métodos: Foram coletadas amostras do exudato de feridas de pacientes com o uso de “Bota de Unna” e de terapia tópica durante a troca do curativo e após sete dias. Os micro-organismos isolados foram identificados e testados quanto à susceptibilidade a antimicrobianos: Resultado: Os micro-organismos Gram positivos isolados foram: S. aureus, E. faecalis, S. xylosus e S. haemolyticus. Os micro-organismos Gram negativos foram: E. coli, P. aeruginosa, S. plymuthica, P. mirabilis, K. pneumoniae, K. oxytoca, P. stuartii, P. vulgaris, A. hydrophila, S. marcescens, A.baumannii, E. cloacae e Tatumella sp. O percentual de crescimento e a microbiota no exudato da úlcera após sete dias não foi significante entre os dois tipos de curativo. O aumento de resistência dos cocos Gram positivos aos antimicrobianos testados nos pacientes que utilizam a “Bota de Unna” foi maior do que nos pacientes sem bota (p=0,0093). Conclusão: O número de micro-organismos na microbiota do exudato da úlcera venosa após troca do curativo é maior independentemente do tipo do curativo. Os cocos Gram-positivos apresentam aumento de resistência aos antimicrobianos nos pacientes que utilizam a “Bota de Unna”.
PURPOSE:To extract the Melaleuca sp. oil and to assess its in vitro inhibitory effect against Staphylococcus aureus isolates obtained from lower limb wounds and resistant to several antibiotics. METHODS:A total of 14 test-tubes containing Mueller-Hinton broth were used to determine the Minimum Inhibitory Concentration (MIC). The following concentrations of the Melaleuca sp. oil were added to the first 11 tubes: 8; 4; 2; 1; 0.5; 0.2; 0.1; 0.05; 0.025; 0.0125 and 0.00625%. The 12 th and 13 th tubes, with and without oil, were used as the positive and negative controls, respectively. The experimental study was carried out in triplicate at 37ºC for 18 hours. The Minimum Bactericidal Concentration (MBC), able of killing all the microorganisms, was also determined. Two S. aureus isolates were obtained from lower limb wounds of female patients and the identification of the microorganisms (Staphylococcus aureus) and the test for susceptibility to the antimicrobial agents were carried out by automation using the apparatus MicroScan ® . After identification, the isolates were preserved in liquid Trypticase Soy medium, and inoculated for determination of the MIC and MBC. RESULTS:The MIC was 0.2% and the MBC was 0.4%. CONCLUSION:The Melaleuca sp. oil showed antimicrobial properties in vitro against strains isolated from lower limb wounds which were resistant to multiple antibiotics.
Introduction: peristomal dermatitis can be caused by leakage between adhesive plate and skin. Objective: to describe the peristomal plate cut mold (PPCM) for simultaneous evaluation of new products for the treatment of dermatitis. Methods: experience report about creation of a cut model, with sufficient size and width to expose part of the skin with dermatitis, to apply another product. The trapezoidal cut, according to positions 3, 6, 9 or 12 hours, corresponding to one of the quadrants, it was performed on the plate of carboxymethylcellulose, pectin, and gelatine coupled to the stoma bag. The entire part of the plate adhered to the skin was adapted to the control product. In the photographic analysis, CorelDRAW 19.0 was used. The lesions were measured in pixels. Result: the cut did not interfere with the adhesion of two parts device board, it made possible the simultaneous application and testing of a new product and the comparison of the healing with the plate. Conclusion: the PPCM is a new strategy for studies of the application of other products for peristomal dermatitis simultaneously in the same patient and demonstrates positive results in its use.
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