The effect of physical variability upon the relative transport behavior of microbial-sized microspheres, indigenous bacteria, and bromide was examined in field and flow-through column studies for a layered, but relatively well sorted, sandy glacioftuvial aquifer. These investigations involved repacked, sieved, and undisturbed aquifer sediments. In the field, peak abundance of labeled bacteria traveling laterally with groundwater flow 6 m downgradient from point of injection was coincident with the retarded peak of carboxylated microspheres (retardation factor, RF = 1.7) at the 8.8 m depth, but preceded the bromide peak and the retarded microsphere peak (RF = 1.5) at the 9.0 m depth. At the 9.5 m depth, the bacterial peak was coincident with both the bromide and the microsphere peaks. Although sorption appeared to be a predominant mechanism responsible for immobilization of microbial-sized microspheres in the aquifer, straining apl•ared to be primarily responsible for their removal in 0.6-m-long columns of repacked, unsieved aquifer sediments. The manner in which the columns were packed also affected optimal size for microsphere transport, which in one experiment was near the size of the small (•-2/•m) groundwater protozoa (flagellates). These data suggest that variability in aquifer sediment structure can be important in interpretation of both small-scale field and laboratory experiments examining microbial transport behavior. of Technol., Cambridge, 1988.

Transport behaviors of unidentified flagellated protozoa (flagellates) and flagellate-sized carboxylated microspheres in sandy, organically contaminated aquifer sediments were investigated in a small-scale (1 to 4-m travel distance) natural-gradient tracer test on Cape Cod and in flow-through columns packed with sieved (0.5to 1.0-mm grain size) aquifer sediments. The minute (average in situ cell size, 2 to 3 m) flagellates, which are relatively abundant in the Cape Cod aquifer, were isolated from core samples, grown in a grass extract medium, labeled with hydroethidine (a vital eukaryotic stain), and coinjected into aquifer sediments along with bromide, a conservative tracer. The 2-m flagellates appeared to be near the optimal size for transport, judging from flowthrough column experiments involving a polydispersed (0.7 to 6.2 m in diameter) suspension of carboxylated microspheres. However, immobilization within the aquifer sediments accounted for a log unit reduction over the first meter of travel compared with a log unit reduction over the first 10 m of travel for indigenous, free-living groundwater bacteria in earlier tests. High rates of flagellate immobilization in the presence of aquifer sediments also was observed in the laboratory. However, immobilization rates for the laboratory-grown flagellates (initially 4 to 5 m) injected into the aquifer were not constant and decreased noticeably with increasing time and distance of travel. The decrease in propensity for grain surfaces was accompanied by a decrease in cell size, as the flagellates presumably readapted to aquifer conditions. Retardation and apparent dispersion were generally at least twofold greater than those observed earlier for indigenous groundwater bacteria but were much closer to those observed for highly surface active carboxylated latex microspheres. Field and laboratory results suggest that 2-m carboxylated microspheres may be useful as analogs in investigating several abiotic aspects of flagellate transport behavior in groundwater.

SummaryAt the Hanford Site in southeastern Washington State, contaminated groundwater discharges to the Columbia River after passing through a zone of groundwater/river water interaction at the shoreline (i.e., the hyporheic zone). In the hyporheic zone, river water may infiltrate the riverbank during periods of high-river stage and mix with the approaching groundwater. Contaminants carried by groundwater may become diluted by the infiltrating river water, thus reducing concentrations at locations of exposure, such as riverbank springs and upwelling through the riverbed. There have been limited studies of contaminant concentrations, physical properties, or the extent of the hyporheic zone near the Hanford Site's 300 Area, yet this zone is a major interface for discharge of groundwater contamination into the Columbia River.The Remediation Task of the Remediation and Closure Science Project conducts research to meet several objectives concerning the discharge of groundwater contamination into the river at the 300 Area of the Hanford Site in Washington State. This report documents research conducted to meet these objectives by developing baseline data for future evaluation of remedial technologies, evaluating the effects of changing river stage on near-shore groundwater chemistry, improving estimates of contaminant flux to the river, providing estimates on the extent of contaminant discharge areas along the shoreline, and providing data to support computer models used to evaluate remedial alternatives. This report summarizes the activities conducted to date, and provides an overview of data collected through July 2006. Recent geologic investigations (funded through other U.S. Department of Energy [DOE] projects)have provided a more complete geologic interpretation of the 300 Area and a characterization of the vertical extent of uranium contamination. Extrapolation of this geologic interpretation into the hyporheic zone is possible, but little data are available to provide corroboration. Penetration testing was conducted along the shoreline to develop evidence to support the extrapolation of the mapping of the geologic facies. While this penetration testing provided evidence supporting the extrapolation of the most recent geologic interpretation, it also provided some higher-resolution detail on the shape of the layer that constrains contaminant movement. Information on this confining layer will provide a more-detailed estimate of the area of riverbed that has the potential to be impacted by uranium discharge to the river from groundwater transport.Water sampling in the hyporheic zone has provided results that illustrate the degree of mixing that occurs in the hyporheic zone. Uranium concentrations measured at individual sampling locations can vary by several orders of magnitude depending on the Columbia River and near-shore aquifer elevations. This report shows that the concentrations of all the measured constituents in water samples collected from the hyporheic zone vary according to the ratio of groundwater and C...

The Department of Energy’s (DOE’s) National Energy Technology Laboratory’s (NETL’s) Blowout and Spill Occurrence Model (BLOSOM), and the National Oceanic and Atmospheric Administration’s (NOAA’s) General NOAA Operational Modeling Environment (GNOME) are compared. Increasingly complex simulations are used to assess similarities and differences between the two models’ components. The simulations presented here are forced by ocean currents from a Finite Volume Community Ocean Model (FVCOM) implementation that has excellent skill in representing tidal motion, and with observed wind data that compensates for a coarse vertical ocean model resolution. The comprehensive comparison between GNOME and BLOSOM presented here, should aid modelers in interpreting their results. Beyond many similarities, aspects where both models are distinct are highlighted. Some suggestions for improvement are included, e.g., the inclusion of temporal interpolation of the forcing fields (BLOSOM) or the inclusion of a deflection angle option when parameterizing wind-driven processes (GNOME). Overall, GNOME and BLOSOM perform similarly, and are found to be complementary oil spill models. This paper also sheds light on what drove the historical Point Wells spill, and serves the additional purpose of being a learning resource for those interested in oil spill modeling. The increasingly complex approach used for the comparison is also used, in parallel, to illustrate the approach an oil spill modeler would typically follow when trying to hindcast or forecast an oil spill, including detailed technical information on basic aspects, like choosing a computational time step. We discuss our successful hindcast of the 2003 Point Wells oil spill that, to our knowledge, had remained unexplained. The oil spill models’ solutions are compared to the historical Point Wells’ oil trajectory, in time and space, as determined from overflight information. Our hindcast broadly replicates the correct locations at the correct times, using accurate tide and wind forcing. While the choice of wind coefficient we use is unconventional, a simplified analytic model supported by observations, suggests that it is justified under this study’s circumstances. We highlight some of the key oceanographic findings as they may relate to other oil spills, and to the regional oceanography of the Salish Sea, including recommendations for future studies.

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