No abstract

No abstract

No abstract

No abstract

Polycystic Ovarian Syndrome (PCOS) is a hyper androgenic condition in a female with persistent oligoanovulation. Between the age of 18 and 44, almost 5% to 10% of females are impacted by this condition. The most prevalent issue in the world is infertility, which is the inability to conceive after a continuous one-year period of exposure to regular, unprotected, typical coitus. Three-quarters to forty percent of all human infertility are female-originated. Therefore, there is a greater demand for PCOS research and awareness. To decrease current treatments' cost, length, and side effects, integrative approaches must be developed based on the aforementioned variables. These approaches can be used to treat the polycystic ovarian syndrome, its consequences, and symptoms like obesity, irregular menstrual cycles, excessive hair growth, etc. This review consolidates current problems, treatment modalities associated with PCOS, and how integrative approaches can handle this syndrome in females.

No abstract

Background:γ-oryzanol is a major bioactive constituent in rice. Most of the literature reports isolation of 24-methylenecycloartanyl ferulate (24-mCAF) from rice bran oil (RBO) of other than Indian variety. Current research has successfully applied high-performance thin layer chromatography (HPTLC) method for isolation of 24-mCAF from Indian variety (Indrayani) of RBO.Materials and Methods:HPTLC method was developed for standard γ-oryzanol using tinidazole as an internal standard. The proposed HPTLC method was optimized and validated as per the guidelines stated by the International Conference on Harmonization Q2 R1 recommendations. The mobile phase composed of toluene:ethyl acetate:methanol (15.0:1.7:3.3, (v/v/v) was selected because well-resolved peaks were obtained. The optimum wavelength chosen for detection and quantitation was 317 nm.Results:The retention factors for tinidazole, 24-mCAF, and CAF were found to be 0.27 ± 0.02, 0.72 ± 0.02, and 0.79 ± 0.02, respectively. The percent content of 24-mCAF in ethanol fraction was found to be 1.02%. The 24-mCAF was isolated from RBO using HPTLC method.Conclusion:The characterization data of 1D, 2D spectral analysis confirm that the isolated compound 1 is 24-mCAF.SUMMARY HPTLC method was developed for standard γ-oryzanol using tinidazole as an internal standardThe proposed HPTLC method was optimized and validated as per the guidelines stated by the ICH Q2 R1 recommendationsThe characterization data of 1D, 2D spectral analysis confirms that the isolated compound is 24-methylenecycloartanyl ferulateIn this work, high purity 24-mCAF was successfully isolated from crude RBO using HPTLC with a solvent system composed toluene: ethyl acetate: methanol (15.0:1.7:3.3, v/v/v) Abbreviations used: RBO: Rice Bran Oil, CAF: Cycloartenol ferulic acid, 24-mCAF: 24-Methylcycloartenol ferulic acid, HPLC: High-Performance Liquid Chromatography, HPTLC: High-Performance Thin Layer Chromatography, 1H: Proton nuclear magnetic resonance spectroscopy, 13C: Carbon-13 nuclear magnetic resonance spectroscopy, COSY: Correlation spectroscopy, NOESY: Nuclear overhauser effect spectroscopy, HMBC: Heteronuclear multiple bond correlation nuclear magnetic resonance spectroscopy, HSQC: heteronuclear single quantum coherence nuclear magnetic resonance spectroscopy.

P38 kinases are the members of serine/ threonine kinases family, play a vital role in the progression of inflammation. In the past two decades, numerous p38 kinase inhibitors have been reported and few of them have failed in clinical trials. Recently some of the p38 kinase inhibitors have entered in clinical trials for the treatment of Alzheimer’s disease. A potential opportunity exists for medicinal chemistry for the discovery of potent and safe p38 kinase inhibitors. In the view of this challenging opportunity, the present manuscript is aimed towards development of a 3D QSAR model and the docking and dynamic simulation studies. A statistically robust 3D QSAR model was developed by employing 21 training set molecules which is attributed with appreciating cross validation coefficient (q2) of 0.0.6269 and conventional correlation coefficient (r2) of 0.8783 respectively. The predicted correlation coefficient (r2 pred) was found to be 0.8644 and standard error of 0.3331. The molecular docking analysis of all the p38 kinase inhibitors revealed that the analogs were well docked into the DFG out pocket of p38 kinase and exhibited hydrogen bond interactions with Asp186 and Lys71. Extension of docking studies to the molecular dynamics simulation study informed that the ligand displayed the strong conformational stability within the active site of p38 kinase forming maximum two hydrogen bonds until 100 ns respectively.