Plant stresses causing accumulation of reactive oxidative species (ROS) are scavenged by effective antioxidant defense systems. Therefore, the present study performed genome-wide identification of superoxide dismutase (SOD) and glutathione peroxidase (GPX) gene families in cultivated and wild soybeans, and 11 other legume species. We identified a total of 101 and 95 genes of SOD and GPX, respectively, across thirteen legume species. The highest numbers of SODs and GPXs were identified in cultivated (Glycine max) and wild (Glycine soja). A comparative phylogenetic study revealed highest homology among the SODs and GPXs of cultivated and wild soybeans relative to other legumes. The exon/intron structure, motif and synteny blocks were conserved in both soybean species. According to Ka/Ks, purifying the selection played the major evolutionary role in these gene families, and segmental duplication are major driving force for SODs and GPXs expansion. In addition, the qRT-PCR analysis of the G. max and G. soja SOD and GPX genes revealed significant differential expression of these genes in response to oxidative, drought and salinity stresses in root tissue. In conclusion, our study provides new insights for the evolution of SOD and GPX gene families in legumes, and provides resources for further functional characterization of these genes for multiple stresses.
BCR-ABL kinase domain (KD) mutations, the most common cause of imatinib resistance, are infrequently detected in newly diagnosed chronic-phase chronic myeloid leukemia (CP-CML) patients. Recent studies indicate pre-existing mutations (PEMs) can be detected in a higher percentage of CML patients using CD34 + stem/progenitor cells, and these mutations may correlate with imatinib resistance. We investigated KD mutations in CD34 + stem cells from 100 CP-CML patients by multiplex ASO-PCR and sequencing ASO-PCR products at the time of diagnosis. PEMs were detected in 32/100 patients and included F311L, M351T, and T315I. After a median follow-up of 30 months (range 8-48), all patients with PEMs exhibited imatinib resistance. Of 68 patients without PEMs, 24 developed imatinib resistance. Mutations were detected in 21 of these patients by ASO-PCR and KD sequencing. All 32 patients with PEMs had the same mutations. In imatinib-resistant patients without PEMs, we detected F311L, M351T, Y253F, and T315I mutations. All imatinib-resistant patients without T315I and Y253F mutations responded to imatinib dose escalation. In conclusion, BCR-ABL PEMs can be detected in a substantial number of CP-CML patients when investigated using CD34 + stem/progenitor cells. These mutations are associated with imatinib resistance, and mutation testing using CD34 + cells may facilitate improved, patient-tailored treatment.
Summary
We report a case of simultaneous malignant hyperthermia reactions occurring in two siblings during living donor liver transplantation. This report highlights the conflicting goals in the clinical management of liver transplantation and malignant hyperthermia, including the use of total intravenous anaesthesia and dantrolene in the face of the potential for drug‐induced hepatotoxicity in the remnant liver or transplanted liver graft, as well as cautious fluid management needed for liver transplantation balanced against the liberal fluid therapy required to prevent acute kidney injury associated with malignant hyperthermia. The logistical challenges of managing this emergency in two closely related patients are discussed, including rapid preparation of two vapour‐free anaesthesia machines, the need for availability of additional dantrolene and the requirement for additional personnel. Prompt recognition, immediate removal of the triggering agents and conversion to total intravenous anaesthesia helped to curtail the malignant hyperthermic reactions in our patients, both of whom made a full recovery.
Introduction
Attitude toward participation in the research plays an important role in the quality of any research. Therefore, researchers aimed at construction and piloting of scale to measure attitude toward research participation for university students.
Method and Results
In Study I, an initial pool of items was generated on the basis of literature review, semi-structured interviews and expert opinions. After ensuring content validity and finalizing items with the help of experts, the scale was applied on a sample of 426 university students including both men (n = 114) and women (n = 312) with age range of 17–30. Exploratory factor analysis revealed the two factor structure was with 60.33% cumulative variance. The factors revealed were positive attitude and negative attitude toward research with alpha reliability of 0.84 for positive attitude while 0.76 for negative attitude. In Study II, confirmatory factor analysis revealed excellent model fit indices with two factor structure. Study III was meant to provide evidence of construct validity. Positive correlation showed exploration and curiosity with positive attitude toward research and negative correlation of exploration and curiosity with negative attitude toward research participation provided strong evidence for convergent validity. While non-significant correlation of attitude toward research with religiosity confirmed the divergent validity of the scale.
Conclusion
Findings of the study revealed that Attitude Toward Research Participation Scale is a valid and reliable measure. It can be used for university students to measure their attitude toward research.
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