Foram realizados dois experimentos para avaliar os efeitos da granulometria do milho das rações, expressa pelo diâmetro geométrico médio (DGM), 0,337; 0,574; 0,680; 0,778; 0,868 e 0,936mm, sobre o desempenho, o metabolismo energético e a morfologia intestinal (número e altura de vilosidades do duodeno) de frangos de corte machos, de 21 aos 42 dias de idade. No 1° experimento, partículas de 0,868 mm proporcionaram às aves maior consumo de ração, maior ganho de peso e melhor conversão alimentar. A altura das vilosidades intestinais não foi influenciada pelo DGM das dietas, mas aumentou com a idade das aves (21, 35 e 42 dias de idade). Não foi observado efeito da granulometria sobre a deposição de gordura abdominal ou sobre as proporções das partes da carcaça. O peso da moela esteve positivamente relacionado com o aumento da granulometria das dietas. No 2° experimento, as aves alimentadas com milho de 0,337 mm apresentaram menor consumo de energia metabolizável e maior excreção de energia bruta. A granulometria não influenciou na retenção de nitrogênio.

In this study, 360 male broilers, out of which 240 of a fast-growing strain (Cobb500), and 120 of a slow-growing strain (Label Rouge), were used to evaluate the effect of dietary fiber on digesta transit time and digestive metabolism during the period of 1 to 42 days of age. A completely randomized experimental design with a 3x2 factorial arrangement was applied, consisting of three groups of birds (slow-growing -SG; fast-growing fed ad libitum -FGAL; and fast-growing pair-fed with SG broilers -FGPF) and two iso-protein diets (a 3100 kcal ME/kg low-fiber diet -LFD-and a 2800 kcal ME/ kg high-fiber diet -HFD-with 14% wheat bran and 4% oat hulls). HFD-fed birds presented lower ME retention (p < 0.001) and lower dry matter metabolizability (DMM) (p < 0.001), which is possibly related to the shorter digesta transit time observed in these birds (p < 0.001). DMM was reduced with age, whereas metabolizable energy remained almost constant (p < 0.001) independently of strain. This may be related to the increase in feed intake as birds age. The slowgrowing strain did not present better utilization of the high-fiber diet as compared to the fast-growing strain in none of the analyzed ages, even though showing a significant better use of fiber and dietary energy from 31 days of age.

The experiment was conducted to evaluate the effect of dietary fiber on the performance and energy balance of broiler chickens of a fast-growing strain (Cobb500) and a slow-growing strain (Label Rokens during the period of 1 one to 42 days of used In total, 360 male broilers (240 fast-grorain and 120 slow-grtrain)were, housed in collective cages. A completely randomized experimental dewith in a 3x2 factorial arrangement was applied, consisting of three groups of birds (slow-growing-SG; fast-growing fed ad libitum-FGAL; and fast-growing pair-fed with SG broilers-FGPF) and two iso-protein dis (a 3100 kcal ME/kg low-fiber diet and a 2800 kcal ME/kg high-fiber diet-Hwith containing 14% wheat bran and 4% oat hulls). Dietary fiber level did not affect feed intake (FI); however, it resulted in lower weight gain (WG) and worse feed conversion ratio (FCR) (p ≤ 0.001) in birds fed the HFD diet due to its lower energy content. The FGPF group presented higher WG than SG and better FCR (p ≤ 0.001), indicating that fast-growing birds present better performance than SG broilers, even under restricted feed intake. The SG group retained more energy relative to body weight (p ≤ 0.001), which is associated to higher body fat retention in this strain (p ≤ 0.001). The slow-growing strain did not present better use of high-fiber diet than fast-growing strain as expected.

The effects of lutein and conjugated linoleic acid (CLA) on growth performance and immune response of broiler chickens were evaluated in the presence and absence of Salmonella lipopolysaccharide (LPS) immune challenge. Cobb chicks (360; 1 to 22 d of age) were used in a 3 × 2 factorial arrangement of CLA (0, 1, and 2%) and lutein (0 and 50 mg/kg) dietary levels. At d 8 and 15, birds were injected with BSA to assess IgY production. At d 20, birds were injected with LPS. Samples of liver, spleen, and duodenum were collected at 3 and 16 h post-LPS challenge for RT-qPCR analysis of RXRα, RXRγ, PPARα, PPARγ, TLR-4, IL-1β, IL-2, IL-10, and IL-12 gene expression. CLA decreased BW, BW gain (BWG), and G:F from d 1 to 20, but these effects were reversed when lutein was included in the 1% CLA diet (P < 0.001). The production of IgY anti-BSA increased following a 2% CLA supplementation (P < 0.01). LPS increased the liver:BW ratio at 3 h post-injection (P < 0.001) and decreased BWG at 3, 16, and 40 h (P < 0.001). Lutein decreased plasmatic nitric oxide levels (P < 0.01). LPS downregulated PPARα mRNA in the duodenum (P = 0.02) and liver (P = 0.04), and PPARγ (P = 0.01) and RXRα (P = 0.08) in the spleen; these effects were not reversed by CLA or lutein as initially hypothesized. Although LPS upregulated IL-1β (P = 0.02) and IL-12 (P = 0.07) expression, lutein downregulated these pro-inflammatory cytokines in the liver (P = 0.03 and P = 0.07, respectively). Lutein decreased splenic (P = 0.09) but increased hepatic (P = 0.06) TLR-4 mRNA. A dietary CLA supplementation of 2% increased hepatic RXRα (P = 0.10). In conclusion, CLA decreased broiler chicken growth performance, but lutein could prevent this negative effect (depending on CLA dose). Lutein had an anti-inflammatory effect, and a 2% CLA supplementation improved the humoral immune response.

Infection with Eimeria sp. results in the activation of multiple facets of the host immune system; the use of phytogenics can modulate the inflammatory response and improve the performance of the challenged animal. The aim of this study was to evaluate the effect of a commercial blend of cashew nut shell liquid (CNSL) and castor oil on the immune response of broilers challenged with coccidiosis. A total of 864 one-day-old male chicks (Cobb 500) were randomly distributed into six treatment groups (8 pens/treatment and 18 chicks/pen) in a three-by-two factorial design with three additives: control (non-additive), 100 ppm of monensin or 0.15% CNSL–castor oil. Challenge status was determined twice at 14 days of age. Unchallenged birds were inoculated by gavage with oocysts sporulated with Eimeria tenella, Eimeria acervulina and Eimeria maxima. Although the positive control (non-additive and challenged) and CNSL–castor oil treatment groups exhibited similar variation in weight gain (ΔBWG) compared to unchallenged birds fed without additives, the variation observed in birds fed diets containing CNSL–castor oil was associated with a higher maintenance requirement and not feed efficiency. In the second week after infection, ΔBWG of the CNSL–castor oil treatment group did not significantly change compared to the other treatment groups. At days 7 and 14 post-challenge, there was a higher excretion of oocysts in the control group, whereas the CNSL–castor oil and monensin groups did not differ. The CNSL–castor oil group exhibited increased gene expression of interferon (IFN), interleukin 6 (IL-6) and tumor necrosis factor (TNF), while the control group exhibited increased expression of cyclooxygenase (COX) and IL-1. The heterophils/lymphocyte ratio was low for the monensin treatment group. The unchallenged birds that received monensin treatment presented higher gene expression of IFN, COX and IL-1 compared to the other treatments, while the CNSL–castor oil group exhibited reduced gene expression, except for TNF. The commercial blend of cashew nut liquid and castor oil modulated the inflammatory response against Eimeria spp. In the absence of the parasite, there was no stimulation of genes involved in the inflammatory response, demonstrating that the blend is an effective tool in specifically modulating the immune system of birds afflicted with coccidiosis.