In order to study the effect of salts stress on the growth and yield of wheat (cv. Inqalab), a pot experiment was conducted in the wire-house of the Department of Soil Science, Sindh Agriculture University Tando Jam. The soil was artificially salinized to a range of salinity levels i.e. EC 2.16, 4.0, 6.0, 8.0 and 10.0 dS•m −1 with different salts (MgCl 2 + CaCl 2 + Na 2 SO 4). The salinized soil used for the experiment was sandy clay in texture, alkaline in reaction (pH > 7.0) and moderate in organic matter (0.95%) content. The results showed that with increasing salinity there was an increase in the ECe, Na + , Ca 2+ , Mg 2+ and Cl − and decrease in the K + , SAR and ESP values of the soil Increasing salinity, progressively decreased plant height, spike length, number of spikelets spike −1 1000 grain weight and yield (straw and grain). Adverse effects of salts on plants were associated with the accumulation of less K + and more Na + and Cl − in their flag leaf sap, grains and straw. This resulted in lower K + :5Na + ratio in flag leaf sap, grains and straw of wheat plants. These results indicated that the effects of salts stress were greater at 10 than at 8, 6 and 4 EC dS•m −1 .

Nitrogen is one of the most important factor affecting maize (Zea mays L.) yield and income of smallholders under semiarid climates. Field experiments were conducted to investigate the impact of different N-fertilizer sources [urea, calcium ammonium nitrate (CAN), and ammonium sulfate (AS)] and rates (50, 100, 150, and 200 kg ha−1) on umber of rows ear−1 (NOR ear−1), number of seeds row−1 (NOS row−1), number of seeds ear−1 (NOS ear−1), number of ears per 100 plants (NOEP 100 plants−1), grain yield plant−1, stover yield (kg ha−1), and shelling percentage (%) of maize genotypes “Local cultivars (Azam and Jalal) vs. hybrid (Pioneer-3025).” The experiment was conducted at the Agronomy Research Farm of the University of Agriculture Peshawar during summers of 2008 (year one) and 2010 (year two). The results revealed that the N treated (rest) plots (the average of all the experimental plots treated with N) had produced higher yield and yield components, and shelling percentage over N-control plots (plots where N was not applied). Application of nitrogen at the higher rate increased yield and yield components in maize (200 > 150 > 100 > 50 kg N ha−1). Application of AS and CAN had more beneficial impact on yield and yield components of maize as compared to urea (AS > CAN > urea). Hybrid maize (P-3025) produced significantly higher yield and yield components as well as higher shelling percentage than the two local cultivars (P-3025 > Jalal = Azam). Application of ammonium sulfate at the rate of 200 kg N ha−1 to hybrid maize was found most beneficial in terms of higher productivity and grower's income in the study area. For the two local cultivars, application of 150 kg N ha−1 was found more beneficial over 120 kg N ha−1 (recommended N rate) in terms of greater productivity and growers income.

The current study aimed to explore the endophytic bacterial diversity of Avicennia marina and the potential roles of these endophytes in counteracting saline conditions in tomato plants. Molecular analysis revealed strains from Paenibacillus, Bacillus, Microbacterium, Citrobacter, Lysinibacillus, Halomonas, Virgibacillus, Exiguobacterium, and Vibrio. However, Bacillus pumilus AM11 and Exiguobacterium sp. AM25 showed significantly higher growth in saline media. In response to salinity stress, tomato plants treated with AM11 and AM25 showed significantly higher (∼15-23%) biomass, photosynthetic rate and pigment accumulation compared to controls. Salinity-exposed plants had significantly reduced growth and increased (three-fold) lipid peroxidation, whilst glutathione, catalase, and peroxidase activities were significantly reduced. In contrast, AM11, AM25, and methionine improved these physiochemical attributes. The study concludes that the application of bacterial endophytes from plants growing in saline conditions can offer other plants similar stress-resistance potential. Such halophytic bacterial strains can be used to improve plant growth in saline conditions. ARTICLE HISTORY

Silver nanoparticles (Ag. NPs) have shown a biological activity range, synthesized under different environment-friendly approaches. Ag. NPs were synthesized using aqueous crude extract (ACE) isolated from Plantago lanceolata. The ACE and Ag. NPs were characterized and assessed their biological and antioxidant activities. The existence of nanoparticles (NPs) was confirmed by color shift, atomic force microscopy (AFM), and UV–Vis’s spectroscopy. The FT-IR analysis indicated the association of biomolecules (phenolic acid and flavonoids) to reduce silver (Ag+) ions. The SEM study demonstrated a sphere-shaped and mean size in the range of 30 ± 4 nm. The EDX spectrum revealed that the Ag. NPs were composed of 54.87% Ag with 20 nm size as identified by SEM and TEM. AFM has ended up being exceptionally useful in deciding morphological elements and the distance across of Ag. NPs in the scope of 23–30 nm. The TEM image showed aggregations of NPs and physical interaction. Ag. NPs formation also confirmed by XPS, DRS and BET studies. Ag. NPs showed efficient activity as compared to ACE, and finally, the bacterial growth was impaired by biogenic NPs. The lethal dose (LD50) of Ag. NPs against Agrobacterium tumefaciens, Proteus vulgaris, Staphylococcus aureus, and Escherichia coli were 45.66%, 139.71%, 332.87%, and 45.54%, with IC50 (08.02 ± 0.68), (55.78 ± 1.01), (12.34 ± 1.35) and (11.68 ± 1.42) respectively, suppressing the growth as compared to ACE. The antioxidant capacity, i.e., 2,2-diphenyl-1-picrylhydrazyl (DPPH) of Ag. NPs were assayed. ACE and Ag. NPs achieved a peak antioxidant capacity of 62.43 ± 2.4 and 16.85 ± 0.4 μg mL−1, compared to standard (69.60 ± 1.1 at 100 μg mL−1) with IC50 (369.5 ± 13.42 and 159.5 ± 10.52 respectively). Finally, the Ag. NPs synthesized by P. lanceolata extract have an excellent source of bioactive natural products (NP). Outstanding antioxidant, antibacterial activities have been shown by NPs and can be used in various biological techniques in future research.

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Conversion of organic waste into engineered metal-biochar composite is an effective way of enhancing biochar’s efficiency for adsorptive capture of phosphorus (P) from aqueous media. Thus, various strategies have been created for the production of metal-biochar composites; however, the complex preparation steps, high-cost metal salt reagent application, or extreme process equipment requirements involved in those strategies limited the large-scale production of metal-biochar composites. In this study, a novel biochar composite rich in magnesium oxides (MFBC) was directly produced through co-pyrolysis of magnesite with food waste; the product, MFBC was used to adsorptively capture P from solution and bio-liquid wastewater. The results showed that compared to the pristine food waste biochar, MFBC was a uniformly hybrid MgO biochar composite with a P capture capacity of 523.91 mg/g. The capture of P by MFBC was fitted using the Langmuir and pseudo-first-order kinetic models. The P adsorptive capture was controlled by MgHPO4 formation and electrostatic attraction, which was affected by the coexisting F− and CO32− ions. MFBC could recover more than 98% of P from the solution and bio-liquid wastewater. Although the P-adsorbed MFBC showed very limited reusability but it can be substituted for phosphate fertiliser in agricultural practices. This study provided an innovative technology for preparing MgO-biochar composite against P recovery from aqueous media, and also highlighted high-value-added approaches for resource utilization of bio-liquid wastewater and food waste. Graphical Abstract

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