Deproteinized whey was used as a substrate for the production of lipids by cold‐adapted yeast Yarrowia lipolytica B9 under non‐sterile culture conditions. Undesired microbial contamination in non‐sterile whey medium could be prevented when appropriate culture parameters (inoculum size of 3 mL/100 mL, initial pH of 5.5 and incubation temperature of 15 °C) were selected. In contrast to additional nitrogen (ammonium sulfate) and phosphorus (potassium dihydrogen phosphate) sources, additional carbon source (lactose) increased lipid accumulation. Under optimized culture conditions, biomass and lipid concentrations of the yeast were found as 7.4 g/L and 4.29 g/L, respectively. Lipid content was determined as 58% of total cell biomass. Fatty acids of the yeast were oleic acid (18:1), cis‐10‐heptadecenoic acid (C17:1), palmitoleic acid (16:1) and palmitic acid (16:0). The yeast was found to contain no polyunsaturated fatty acids. The content of C16 and C18 fatty acids was found to be 91.98% of total lipids. Monounsaturated fatty acids accounted for 80.54% of total lipids. Due to rich monounsaturated fatty acid composition, biomass of Y. lipolytica B9 may be used as feedstock for biodiesel production, especially operating in winter conditions. This is the first report on the use of cheese whey as a lipid production substrate for cold‐adapted microorganisms including Y. lipolytica yeast. Besides, lipid production potential of Y. lipolytica under non‐sterile culture conditions was investigated for the first time in this study. © 2015 Society of Chemical Industry and John Wiley & Sons, Ltd

The objective of this work was to perform production of L-lactic acid from starch-rich waste loquat kernels by newly isolated Rhizopus oryzae MBG-10 fungus. Loquat kernel flour (LKF) was used as substrate (mainly as carbon source). The most favorable conditions for L-lactic acid production were LKF concentration of 80 g/L, CaCO 3 concentration of 20 g/L, ammonium sulfate concentration of 3 g/L and incubation time of 108 h. Under these conditions, L-lactic acid and biomass concentrations were 45.4 and 8.2 g/L, respectively, and a-amylase activity was 81.6 U/mL. No significant pH changes were observed in the medium thanks to the buffering capacity of LKF. L-lactic acid could be produced in a single-stage from starch-rich LKF without prior saccharification by the fungus with high amylolytic enzyme activity. This is the first report on use of waste loquat kernels as a lactic acid production substrate.