Urinary tract infections (UTIs) are one of the most commonly presented infections among men and women as they acquire it at least once in their lifetime and disease can recur. In recent era, one of the challenges faced by humans is progressively increasing dissemination of antimicrobial resistance among pathogens causing UTIs and other diseases. A hospital-based investigation was carried out including 200 UTI patients affirmed by clinicians. Samples selected based on initial screening by nitrite test were cultured and gram-stained. 161 isolates of gram-negative bacteria were characterized based on morphological and biochemical analysis. Antibiotic susceptibility testing was done by Kirby-Bauer disc diffusion method against 16 selected antibiotics. Genotyping was done for blaTEM and blaCTX-M by PCR. Out of the 161 isolated gram-negative bacteria, E. coli (N=116, 72%) was the most common followed by Klebsiella oxytoca (N=22, 13%), Klebsiella pneumoniae (N=14, 9%), Proteus mirabilis (N=6, 4%) and Proteus vulgaris (N=3, 2%). Antibiotic susceptibility testing revealed that isolated gram-negative pathogens were highly sensitive to Amikacin, Fosfomycin, Imipenem and Meropenem where as high level of resistance was observed against Ampicillin, Amoxicillin, Cefotaxime, Nalidixic acid and Norfloxacin. The blaTEM and blaCTX-M genotyping showed that around half of the isolates were positive for either or both of these genes. blaCTX-M (57%) was described as being more common as compared to blaTEM (45%). Pertinent to the rapidly evolving drug resistance patterns amongst pathogenic bacteria, conducting monitoring and surveillance studies to provide updates to physicians regarding latest and emerging trends of drug resistance is crucial globally.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.