Five phenanthrene and two dihydrophenanthrene derivatives were isolated from the diethyl ether extract of fresh rhizomes of Dioscorea communis (L.), among them a phenanthrentriol 1 reported for the first time from Dioscoreaceae family and two dihydrophenanthrene derivatives 6 and 7 reported also for the first time from Dioscorea species. The structures of isolated compounds were elucidated using UV, IR, 1D-, 2D-NMR, and MS techniques. The anticholinesterase activity of extracts and four compounds was evaluated for the first time against acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzymes using Ellman method. Moreover, the antioxidant activity of extracts and three compounds has been investigated using DPPH radical scavenging, ABTS cation radical decolorization, CUPRAC, reducing power and β-carotene bleaching assays.
Objective: The aim of this study was the isolation and identification of secondary metabolites from Crataegus azarolus (L.) and the antioxidant evaluation of its extracts and compounds.Methods: The air-dried powdered parts of the plant were extracted with 70% methanol and fractionated by chloroform, ethyl acetate, and n-butanol. The n-butanol extract was separated using polyamide SC6 column and silica gel TLC. In addition, a fraction of silica gel column of the CHCl3 extract was analyzed by gas chromatography–mass spectrometer (GC–MS). The total phenolic and total flavonoid contents of CHCl3 and n-butanol extracts were estimated. Furthermore, the antioxidant activities of CHCl3, n-butanol extracts, and two flavonoids were evaluated according to five different methods.Results: Eight compounds were identified in CHCl3 and n-butanol extracts, among them, five volatile compounds identified by GC–MS for the 1st time from the species, as well as three known flavonol glycosides identified by spectral analysis (ultraviolet,1H-nuclear magnetic resonance [NMR], and13C-NMR) and by comparison with literature data. The n-butanol extract showed the higher content of polyphenols (307.33 ± 2.33 mg (gallic acid equivalents)/g extract) and flavonoids (143.0 ± 2.12 mg QE/g extract) and it proves the highest antioxidant activity with all assays used.Conclusion: Five volatile compounds were identified for the 1st time from the C. azarolus and the antioxidant potential of plant extracts was measured using five different methods.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.