Aging strongly delays the immunity. Our research aims to assess the in vitro effects of royal jelly (RJ) on the immune function of aged PBMCs. PBMCs were obtained from 10 healthy aged and young donors by the gradient density centrifugation method and further cultured in RPMI-1640 medium supplemented with or without RJ in the presence of Con A. Cell proliferation was assessed by MTT assay along with the measurement of interleukins, Nitric oxide (NO), Glutathione (GSH), and Malondialdehydes (MDA). Our results showed that RJ improved PBMCs proliferation significantly in the elderly subjects, accompanied by the increase in NO (p = .001) and the release of IL-2, IL-4, and IL-6 cytokines. RJ also increased the intracellular GSH (p = .001) and MDA (p = .001) levels in aged PBMCs. In young subjects, RJ enhanced PBMCs proliferation potency, IL-4, IL-6, GSH, and intracellular MDA levels but with a concomitant decrease in NO and IL-2 cytokine secretion as compared with non RJ-treated cells. In conclusion, RJ restored functions of the aged PBMCs as well as the young control subjects, indicating a beneficial effect on immune status during the aging process. Practical applications Royal jelly is a well-known edible dietary compound, used traditionally to treat many diseases throughout the world. Since antiquity, it was shown to have medicinal importance. The immuno-enhancing potential of this food was largely and scientifically established by the lipid and protein fractions. The present study illustrates the antiaging and stimulatory effects of the fresh RJ whole extract, from local Algerian honey bee: Apis mellifera intermissa, on the immunity of aged men. This study provides the experimental evidence supporting anti-immunosenesence effects of royal jelly. RJ supplementation can be used in the old age management and human age-related complications, especially, associated with the weaknesses of the immune response.
Immunosenescence, oxidative stress, and low vaccine efficacy are important symptoms of aging. The goal of our study was to test if quercetin had antiaging and stimulating effects on peripheral blood mononuclear cell (PBMC) immune cells in vitro in the presence of concanavalin a, PBMCs were isolated from healthy elderly and young people and cultured in a complete Roswell Park Memorial Institute 1640 medium supplemented with quercetin. Cell proliferation was assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide colorimetric assay after a 48-h incubation period. Spectrophotometric assays were used to assess oxidative biomarkers (proteins carbonyl [PC], malondialdehydes [MDA], and reduced glutathione [GSH]). The enzyme-linked immunosorbent assay method was used to determine the amount of interleukin (IL)-2 released. A Griess reagent was used to investigate inducible nitrite oxide synthase (iNOS) activity. When compared to young control cells, aged PBMCs had lower proliferation potency, lower IL-2 and NO release, and higher MDA and PC levels. Importantly, quercetin-treated aged PBMCs have a high proliferative response comparable to young cells, restored iNOS activity, and increased levels of GSH antioxidant defences. In comparison to untreated aged PBMCs, treated PBMCs have lower lipo-oxidative damage but higher PC levels.Quercetin may be used as a promising dietary vaccinal adjuvant in the elderly, it has significant effects in reducing immunosenescence hallmarks, as well as mitigating the lipo-oxidative stress in PBMCs cells.
Background: Aging is associated with immunity decline and low vaccinal efficacy. The purpose of our research was to evaluate the possible in vitro stimulating effects of quercetin combined with concanavalin a on PBMCs immune cells. Methods: PBMCs from healthy aged and young individuals were obtained by the gradient density centrifugation. PBMCs were cultured at a 96-well cell culture microplate in RPMI-1640 medium supplemented with quercetin in the presence of concanavalin a and incubated in a 5% CO2 humidified incubator at 37 C for 48 h. Cell proliferation was assessed with MTT colorimetric assay. Intracellular levels of MDA, carbonyl proteins, glutathione were assessed by spectrophotometric assays. IL-2 release was determined by ELISA method. iNOS activity was studied by measuring nitrite products levels using Griess reagent. Results: As compared to their respective young controls; aged PBMCs showed a low proliferation potency and low IL-2 and NO release, while intracellular Malondialdehydes (MDA), carbonyl proteins were higher in these cells. Importantly, PBMCs from aged subjects treated by quercetin and con A display significantly a high proliferative response similar to young cells, a restored iNOS activity and a reduced cellular oxidative damages with mitigated MDA formation but with high CP levels compared to untreated PBMCS cells (data not shown). Conclusion: According to our results, it can be concluded that quercetin combined with Con a lectin may be a safe promising vaccinal adjuvant and it has significant effects in reducing the complications of aging in immune cells , as well as mitigating the oxidative stress in PBMCs cells.
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