Background: Paclitaxel-induced neuropathy still remains a clinical problem for patients undergoing chemotherapy. Objective: The objective of the study was to determine the interaction between xylopic acid and pregabalinco-administration as well as their toxicity in paclitaxel-induced neuropathy. Materials and Methods: Neuropathic pain was induced in rats with 2 mg/ kg of paclitaxel on four alternate days (days 0, 2, 4 and 6). Equi effective doses of xylopic acid and pregabalin that produced 50% anti-nociception (ED 50 ) were determined from their log-dose response curves in the cold allodynia and thermal pain tests. Xylopic acid and pregabalin were again administered to rats in a fixed ratio combination (1:1) of their ED 50 's in order to determine the experimental ED 50 (Z exp ) of the co-administered compounds. Isobolograms were constructed to compare the Z exp to a theoretical ED 50 (Z add ). Blood samples from the various treatment groups of rats were collected for toxicological assessment of the co-administered compounds. Results: The Z exp lay below the Z add on the isobologram of the cold allodnia test. The co-administration exhibited additivity in the thermal pain test. The co-administration did not produce significant (p>0.05) toxicity in rats. The co-administration may be beneficial in paclitaxel-induced neuropathy.Key words: Isobolograms, Neuropathic pain, Paclitaxel, xylopic acid, Toxicity. SUMMARY• Paclitaxel-induced neuropathy is still a clinical problem.• Pregabalin and Xylopic acid co-administration produced anti-nociceptive synergism against this pain type.• The co-administration was fairly safe in animals. PICTORIAL ABSTRACTAbbreviations used: XA: Xylopic acid, PG: Pregabalin, Z add : Theoritical ED 50 , Z exp : Experimental ED 50 .
Traditionally, Aspilia africana is used in the management of pain in Ghana and most parts of West Africa. This study therefore investigated the analgesic effect of the petroleum ether, aqueous, and hydro-ethanolic leaf extracts of Aspilia africana using rodent models. Preliminary phytochemical screening was done on all the extracts, which showed the presence of alkaloids, flavonoids, saponins, tannins, glycosides, phytosterols and terpenoids. The extracts (40-400 mg/kg p.o.) were administered to Sprague-Dawley rats and tail flick latencies (Tail flick analgesic model) were measured in a preliminary analgesic study. The order of analgesic efficacy established was hydro-ethanolic > aqueous > petroleum ether extract. Thin layer and high performance liquid chromatographic analyses were carried out on the hydro-ethanolic extract to obtain chromatograms as fingerprints for identification purposes. These revealed seven spots (TLC) and two peaks (HPLC). Acetic acid-induced writhing and Capsaicin-induced nociception analgesic tests were carried out in ICR mice using the hydroethanolic leaf extract. This significantly (P ≤ 0.001) and dose-dependently suppressed the time-course of acetic acid-induced writhing and capsaicin-induced nociception similar to 10 mg/kg Diclofenac sodium (P ≤ 0.001) and 5 mg/kg, Ketamine (P ≤ 0.001). In conclusion, the leaf extracts of Aspilia africana has significant analgesic activity with the hydro-ethanolic extract being the most potent.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.