An experiment was conducted to determine the efficiency of banana peel to reduce the toxic effect of aflatoxins (AFS) in male albino rats. Seventy eight male albino rats were assigned to 13 experimental diets. The first one served as control received basal diet only, the second served as control DMSO (basal diet and 5ml DMSO /kg B.W.) daily, but the third and fourth one were received 1mg and 2mg AFs/ml respectively. The three following groups were received basal diet only supplemented with banana peel in percentage of 1,2 and 3%,then three groups received basal diet and orally (1mg AFS suspended in 5ml DMSO /kg B.W.) low dose of aflatoxins with the same levels of banana peel. The last three groups received basal diet and orally (2mg AFS suspended in 5ml DMSO /kg B.W.) high dose of aflatoxins with the same levels of banana peel .The duration of this experiment was 6 weeks. Results showed that, albino rats received 38mg and 76mg/kg aflatoxins revealed a significant elevation in the serum levels of AST, ALT, ALP, creatinine, urea, cholesterol and triglyceride, while decrease in total protein (TP) and albumin. Results of hematological parameters showed decrease in red blood cells (RBCs), hemoglobin (HB), packed cell volume (PCV), mean Corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and mean corpuscular hemoglobin (MCH) and platelets while increase in total leucocyte count (TLC).Body weights and organ weights were determined to study its changes. The treatments with banana peel can play a practical role in detoxification of aflatoxins in albino rats. G3Received orally low dose of AF S (1mg AFS suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday G4 Received orally high dose of AF S (2mg AFS suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday G5 Received basal diet supplemented with banana peel at level 1% G6 Received basal diet supplemented with banana peel at level 2% G7 Received basal diet supplemented with banana peel at level 3% G8 Received orally low dose of AF S (1mg AF S suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday +1% banana peel G9 Received orally low dose of AF S (1mg AF S suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday +2% banana peel G10 Received orally low dose of AF S (1mg AF S suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday +3% banana peel G11 Received orally high dose of AF S (2mg AF S suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday +1% banana peel G12 Received orally high dose of AF S (2mg AF S suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday +2% banana peel G13 Received orally high dose of AF S (2mg AF S suspended in 5ml DMSO/ kg B.W.) twice/ week on Monday and Thursday +3% banana peel
This work was conducted to investigate the effect of ethanolic pomegranate peels (PP) extract on liver and kidney structure and function in rats experimentally exposed to different doses of aflatoxins (AFS). An experiment was conducted for 6 weeks, 36 male rats were divided into 6 groups (6 rats in each). G1 served as vehicle treated control, G2 received PP extract via gavage (50 mg/kg body weight (b.w.) daily). G3 received AFS via gavage (1 mg/kg b.w.) twice/week. G4 received AFS via gavage (2 mg/kg b.w.) twice/week. G5 received AFS twice/week (1 mg/kg b.w.) + (PP) extract daily (50 mg/kg b.w.). G6 received AFS twice/week (2 mg/kg b.w.) + (PP) extract daily (50 mg/kg b.w.). Liver enzymes (Alanine Amino Transferase (ALT), Aspartate Amino Transferase (AST), Alkaline Phosphatase (ALP)), Total Protein (TP), albumin and renal biochemical parameters (creatinine, urea and uric acid) were estimated in serum at 3 rd and 6 th week post treatment , while pathological examination of liver and kidney was performed at the end of experiment. Result demonstrated that both aflatoxins administrated levels caused elevation in AST, ALT, ALP, creatinine, urea and uric acid and a decrease in TP and albumin in dose and time dependant manner, while synergistic administration of PP extract and AFS resulted in limitation of the negative effects of AFS on the tested parameters especially with low AFS dose and longer time. Pathological examination revealed that the severity of damage in liver and kidney correlated with aflatoxins , doses. While PP extract administration was able to protect liver and kidney against low AFS dose and minimize the damage of liver and kidney induced by high AFS dose.
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