Background and Objectives In Hong Kong, the dominant circulating hepatitis E virus (HEV) genotype is type 4, which can cause more severe clinical consequences than type 3. The aim of this study was to determine the HEV prevalence in Hong Kong blood donors. Materials and MethodsUnlinked donation samples (n = 10 000) collected in March to May 2015 were tested for HEV RNA using the Procleix HEV assay in an individual donation format (IDT). A subset of 2000 samples were tested for IgG and IgM anti-HEV using the Wantai enzyme-linked immunosorbent assay (ELISA). Nucleic acid testing (NAT) initial reactive results were retested once, and repeatedly reactive donations were subjected to alternative molecular procedures as confirmation tests.Results One in 5000 Hong Kong blood donors was positive for HEV RNA (0Á02%). The two RNA positive samples were also IgG and IgM anti-HEV positive. One of the two RNA positive donors could be sequenced revealing genotype type 4. Anti-HEV seroprevalence was estimated as 15Á5% among all donors. IgG anti-HEV positive rate for age group 16-20 was 3Á1%, and it increased with age to 43Á1% for age group 51-60. Sero-positivity was higher in males (male donors 18Á1% vs. female donors 13Á2%), but it was mostly due to the difference in a specific age group (41-50).Conclusion Hepatitis E virus RNA positive rate of 0Á02% was within the reported range of HEV RNA frequency in developed countries. One donor was confirmed to be genotype 4, which is the dominant genotype in circulation in Hong Kong.