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The aim of this study was to evaluate different formulations of a biodegradable black concentrate obtained from cassava starch and carbon black, on the mechanical properties, color, and water absorption of a film destined to the production of biodegradable bags for coffee seedlings. The modulus of elasticity, tensile strength and elongation properties is found to show significant variation due to the pigment, plasticizer, and lubricant concentration, both longitudinally and transversely of the film, the treatment being 40% pigment with the absence of plasticizer and lubricant for the pigment, which presented greater integrity in the mechanical properties evaluated in both directions. It is also noticeable that the different masterbatch formulations had an influence on the changes in the color parameters and weight gain of the film by the influence of water absorption.