Goal-directed actions are guided by expected outcomes of those actions. Humans with bilateral damage to ventromedial prefrontal cortex, or the amygdala, are deficient in their ability to use information about positive and negative outcomes to guide their choice behavior. Similarly, rats and monkeys with orbital prefrontal or amygdala damage have been found to be impaired in their responses to changing values of outcomes. In the present study, we tested whether direct, functional interaction between the amygdala and the orbital prefrontal cortex is necessary for guiding behavior based on expected outcomes. Unlike control monkeys, rhesus monkeys with surgical disconnection of these two structures, achieved by crossed unilateral lesions of the amygdala in one hemisphere and orbital prefrontal cortex in the other, combined with forebrain commissurotomy, were unable to adjust their choice behavior after a change in the outcome (here, a reduction in the value of a particular reinforcer). The lesions did not affect motivation to work for a food reinforcer, or food preferences, per se. Hence, the amygdala and orbital prefrontal cortex act as part of an integrated neural system guiding decision-making and adaptive response selection.
Lateral organization of cholesterol in dioleoyl-phosphatidylcholine (DOPC) lipid bilayers at high cholesterol concentration (>45 mol%) was investigated using steady-state fluorescence anisotropy and fluorescent resonance energy transfer techniques. The recently devised Low Temperature Trap method was used to prepare compositionally uniform cholesterol/DOPC liposomes to avoid the problem of lipid demixing. The fluorescence anisotropy of diphenylhexatrience chain-labeled phosphatidylcholine (DPH-PC) in these liposomes exhibited local maxima at cholesterol mol fractions of 0.50 and 0.57, and a sharp drop at 0.67. For the liposomes labeled with both dehydroergosterol and DPH-PC, the fluorescent resonance energy transfer efficiency from dehydroergosterol to DPH-PC displayed a steep jump at cholesterol mol fraction of 0.5, and dips at 0.57 and 0.68. These results indicate the presence of highly ordered cholesterol regular distribution domains at those observed critical compositions. The observed critical mol fraction at 0.67 agreed favorably with the solubility limit of cholesterol in DOPC bilayers as independently measured by light scattering and optical microscopy. The regular distribution at 0.57 was previously predicted from a Monte Carlo simulation based on the Umbrella model. The results strongly support the hypothesis that the primary requirement for cholesterol-phospholipid mixing is that the polar phospholipid headgroups need to cover the nonpolar body of cholesterol to avoid the exposure of cholesterol to water.
It is well established that the responses of neurons in the
lateral geniculate nucleus (LGN) can be modulated by feedback
from visual cortex, but it is still unclear how cortico-geniculate
afferents regulate the flow of visual information to the cortex
in the primate. Here we report the effects, on the gain of LGN
neurons, of differentially stimulating the extraclassical receptive
field, with feedback from the striate cortex intact or inactivated
in the marmoset monkey, Callithrix jacchus. A horizontally
oriented grating of optimal size, spatial frequency, and temporal
frequency was presented to the classical receptive field. The
grating varied in contrast (range: 0–1) from trial to
trial, and was presented alone, or surrounded by a grating of
the same or orthogonal orientation, contained within either
a larger annular field, or flanks oriented either horizontally
or vertically. V1 was ablated to inactivate cortico-geniculate
feedback. The maximum firing rate of LGN neurons was greater
with V1 intact, but was reduced by visually stimulating beyond
the classical receptive field. Large horizontal or vertical
annular gratings were most effective in reducing the maximum
firing rate of LGN neurons. Magnocellular neurons were most
susceptible to this inhibition from beyond the classical receptive
field. Extraclassical inhibition was less effective with V1
ablated. We conclude that inhibition from beyond the classical
receptive field reduces the excitatory influence of V1 in the
LGN. The net balance between cortico-geniculate excitation and
inhibition from beyond the classical receptive field is one
mechanism by which signals relayed from the retina to V1 are
controlled.
Macaque monkeys learned a strategy task in which two groups of visual objects needed to be treated differently, one with persistent and one with sporadic object choices, to obtain food rewards. After preoperative training, they were divided into two surgical groups of three monkeys each. One group received crossed unilateral removals of frontal cortex and inferior temporal cortex (IT x FC) and were severely impaired in performing the strategy task. The other group received bilateral transection of anterior temporal stem, amygdala, and fornix (TS+AM+FX) and were unimpaired in performing the strategy task. Subsequently the same animals were tested in visual object-reward association learning. Here, confirming previous results, group IT x FC was unimpaired, whereas group TS+AM+FX was severely impaired. The results show that the amnesic effects of TS+AM+FX cannot be generally attributed to the partial temporal-frontal disconnection that this lesion creates, and therefore support the hypothesis that the amnesic effects of this lesion are caused primarily by the disconnection of temporal cortex from ascending inputs from the basal forebrain. The results also show that temporal-frontal interaction in strategy implementation does not require those routes of temporal-frontal interaction that are interrupted in TS+AM+FX, and therefore support the hypothesis that projections to other posterior cortical areas allow temporal and frontal cortex to interact with each other by multisynaptic corticocortical routes in strategy implementation.
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