Astrocytomas account for the majority of malignant brain tumors diagnosed in both adult and pediatric patients. The therapies available to treat these neoplasms are limited, and the prognosis associated with high-grade lesions is extremely poor. Mer (MerTK) and Axl receptor tyrosine kinases (RTK) are expressed at abnormally high levels in a variety of malignancies, and these receptors are known to activate strong antiapoptotic signaling pathways that promote oncogenesis. In this study, we found that Mer and Axl mRNA transcript and protein expression were elevated in astrocytic patient samples and cell lines. shRNA-mediated knockdown of Mer and Axl RTK expression led to an increase in apoptosis in astrocytoma cells. Apoptotic signaling pathways including Akt and extracellular signal–regulated kinase 1/2, which have been shown to be activated in resistant astrocytomas, were downregulated with Mer and Axl inhibition whereas poly(ADP-ribose) poly-merase cleavage was increased. Furthermore, Mer and Axl shRNA knockdown led to a profound decrease of astrocytoma cell proliferation in soft agar and a significant increase in chemosensitivity in response to temozolomide, carboplatin, and vincristine treatment. Our results suggest Mer and Axl RTK inhibition as a novel method to improve apoptotic response and chemosensitivity in astrocytoma and provide support for these oncogenes as attractive biological targets for astrocytoma drug development.

Purpose -This paper aims to appraise the delivery of an enterprise education course to a cohort of Polish students evaluating its impact in encouraging entrepreneurial activity. The Polish economy continues its expansion with adoption of free market economies post communism. To encourage this growth, entrepreneurial activity must be encouraged within the next generation of entrepreneurs namely the student community. The course entitled Starting a New Enterprise (SANE) was developed to provide entrepreneurial skills and knowledge of the business planning process. The enterprise education literature questions its effectiveness in encouraging entrepreneurial activity. This study profiles the SANE course focusing on students entrepreneurial motivations, prior experiences and future intent. Design/methodology/approach -This study presents a quantitative review of the Polish students' reflections on the experience of enterprise within the SANE course. The basis for this investigation involved two semi-structured questionnaires undertaken prior to and on completion of the course. In total, 59 students completed the first questionnaire and 50 respondents the second. Findings -The study found that Polish students had limited prior entrepreneurial experiences and expectations and welcomed the opportunity to undertake enterprise education. The findings suggested an equal proportion of male and female students aged 18-24 favoured a future entrepreneurial career. Moreover, a quarter of all respondents welcomed an immediate entrepreneurial career on graduation and found value in the development of a business proposal. The findings suggested that entrepreneurial education informs entrepreneurial intent and career aspirations. Originality/value -This study provides evidence into the effectiveness of enterprise education courses as a mechanism to encourage nascent entrepreneurial activity.

Specimens and associated data in natural history collections (NHCs) foster substantial scientific progress. In this paper, we explore recent contributions of NHCs to the study of systematics and biogeography, genomics, morphology, stable isotope ecology, and parasites and pathogens of mammals. To begin to assess the magnitude and scope of these contributions, we analyzed publications in the over the last decade, as well as recent research supported by a single university mammal collection (Museum of Southwestern Biology, Division of Mammals). Using these datasets, we also identify weak links that may be hindering the development of crucial NHC infrastructure. Maintaining the vitality and growth of this foundation of mammalogy depends on broader engagement and support from across the scientific community and is both an ethical and scientific imperative given the rapidly changing environmental conditions on our planet.