A catalogue of the Opiliones types of the "Instituto Butantan", São Paulo, Brazil is given, surveying the collection after severe fire damaged in 2010. Of a total of 91 species with type material listed for the collection, 69 could be located, and 22 are considered lost. The species are arranged according to their families and genera. The collection of Salvador de Toledo Piza Jr., housed at the Museu de Zoologia "Luiz de Queiroz", was donated to the Instituto Butantan in 2009. These types received a new accession number and are listed under this new affiliation for the first time.
The genus Metalibitia Roewer, 1912, is revised for the first time, and a cladistic analysis revealed that it is a monophyletic clade. This cosmetid harvestmen genus is restricted to open forests and adjacent vegetational formations in South America (humid Forest, being found mostly in the region of cerrado/chaco/pampas). An identification key for all known species is presented and a new species is described, Metalibitia abuna sp. nov. (type locality: Abunã, Porto Velho, Rondônia, Brazil). Two species, Paralibitia maculata Roewer, 1914 and Labrosa arenosa Mello-Leitão, 1939, are here synonymized under Metalibitia argentina (Sørensen, 1884).
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