A simple, sensitive, economic and specific reverse phase liquid chromatographic method was developed for the simultaneous estimation of Emtricitabine and Ritonavir in bulk drug. Chromatographic conditions consisted of C-18 Column (Shim-pack) 250 x 4.6 mm, particle size 5 µm , mobile phase combination of methanol and water (80:20), flow rate 1ml per minutes, run time 15 minutes and UV detection at 251nm. . The retention time for Emtricitabine and Ritonavir were found to be 3.25 and 7.8 min and average percentage recoveries 99.42% and 99.63% respectively. The validation parameters were found to comply with ICH guidelines. These methods can be further employed in future for the routine determination of Emtricitabine and Ritonavir in bulk drug and formulation. Keyword: Emtricitabine, Ritonavir, RP-HPLC, accuracy and linearity.
LC-TOF-MS is powerful analytical technique. It is a combination of two techniques one of which is belongs to chromatography and other is from spectroscopy. Chromatography is separation technique and perform both techniques separately it is very time consuming but combined both techniques to save time and provide better results. This represents the potential of liquid chromatography with (quadrupole) time-of-flight mass spectrometry [LC-(Q)TOF-MS] in examining the presence of pesticide metabolites in food and water samples. This method portrays a quick enhanced screen for blood and urine specimens in post-mortem, driving under the influence and drug facilitated sexual assault forensic toxicology casework. (LC–MS) is an analytical technique that amalgamate the physical separation capability of liquid chromatography with the mass analysis capability of mass-spectrometry (MS).This technique can be used in analysis of pharmacokinetics, proteomics/metabolomics, development drug, analysis of pesticides in vegetables, analysis of medicinal panaxherbs for metabolomic research, identification of diphenhydramine in segment sample, investigation of pesticides metabolites in food and water etc.
The hyphenated technique is developed from the coupling of a separation technique and an on-line spectroscopic detection technology, GCMS, LC-MS, LC-FTIR, LC-NMR, and CE-MS. Gas chromatography combined with mass spectrometry is an important technique for identification and quantification of analytes in multifactor systems. GC-MS is highly effective and versatile analytical techniques with numerous scientific applications to cater the field of applied Sciences and Technology. This review elaborates the significant uses of this technique. It includes a brief discussion of the instrumental set-up and theory for the comprehensive GC × GC hyphenated with different detection techniques. It is fast and sensitive, provides a high peak capacity, and allows determination of thermally stable and volatile compounds.
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